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Series GSE3401 Query DataSets for GSE3401
Status Public on Nov 05, 2005
Title 4Y vs 1-11 combined CD4 sort
Organism Mus musculus
Experiment type Expression profiling by array
Summary Local catabolism of the amino acid tryptophan (Trp) by indoleamine-2,3-dioxygenase (IDO) is considered an important mechanism of regulating T cell immunity. We show that IDO transcription was increased upon stimulation of myelin-specific T cells with tolerogenic altered self-peptides. Catabolites of Trp suppressed proliferation of myelin-specific T cells and inhibited production of proinflammatory TH1 cytokines. N-(3,4,-dimethoxycinnamoyl) anthranilic acid (3,4-DAA), an orally active synthetic derivative of the Trp metabolite anthranilic acid, reversed paralysis in mice with experimental autoimmune encephalomyelitis, a model of multiple sclerosis (MS). Trp catabolites and their derivatives offer a new strategy for treating TH1-mediated autoimmune diseases, such as MS.

4Y vd 1-11 comparison files are available as supplementary files.
Keywords: 48hr activation, MBPAc1-11 Cd4+ T cell, murine Mu11ksubA and Mu11subB combined anaylsis
Overall design Double stranded cDNA was generated from total RNA extracted from MBP Ac 11-11 activated purified CD4+ T cells. Biotinylated cRNA was manufactured by in vitro transcription and hybridized to the Affymetrix genechip murine Mu11k set according to the manufacturer's protocols. Results were calculated as the ratio of mean average differences for each gene in 4Y versus Ac1-11 stimulated samples.
Contributor(s) Steinman L, Platten M, Ho PP, Garren H
Citation(s) 16272121
Submission date Sep 30, 2005
Last update date Jul 08, 2016
Contact name Lawrence Steinman
Phone 650-725-6401
Organization name Stanford University School of Medicine
Department Neurology and Neurological Sciences
Street address 279 Campus Drive, Rm B002
City Stanford
State/province CA
ZIP/Postal code 94305-5316
Country USA
Platforms (2)
GPL75 [Mu11KsubA] Affymetrix Murine 11K SubA Array
GPL76 [Mu11KsubB] Affymetrix Murine 11K SubB Array
Samples (2)
GSM76659 1-11Mu11ksubA_48hrTCL
GSM76660 1-11 Mu11KsubB_48hr TCL
BioProject PRJNA93533

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3401_4Yvs_1-11Mu11ksubA_48hrTCL.txt 734.1 Kb (ftp)(http) TXT
GSE3401_4Yvs_1-11Mu11ksubB_48hrTCL.txt 757.3 Kb (ftp)(http) TXT

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