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| Status |
Public on Feb 27, 2012 |
| Title |
Microarray analysis of the migrating human CD4+ T cell population from the spleen of humanized mice in response to treatment with Teplizumab or hIg |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to identify genes expressed by cells that leave the spleen, the spleens were harvested from untreated reconstituted humanized mice (N=4) and a single cell suspension was prepared . The cultures were treated with either teplizumab (anti human CD3 hOKT3g1(Ala-Ala)) or hIg for 18 hrs in vitro. Splenocytes were also harvested 18 hrs after reconstituted mice (N=4) were treated with teplizumab in vivo. The humanized mice used where NOD/SCID IL2gc-/- (NSG) reconstituted with human CD34+ at birth.
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| |
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| Overall design |
Total RNA was obtained form sorted human CD4 splenocytes 18 hours post treatment. Comparsons were made between the treatment groups.
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| |
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| Contributor(s) |
Waldron-Lynch F, Herold K |
| Citation(s) |
22277969 |
| |
| Submission date |
Dec 05, 2011 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Frank Waldron-Lynch |
| Organization name |
Yale University Medical School
|
| Department |
Human Translational Immunology
|
| Street address |
300 George Street
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
|
| Samples (11)
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| Relations |
| BioProject |
PRJNA149707 |
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