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Series GSE34680 Query DataSets for GSE34680
Status Public on Feb 01, 2012
Title Effects of Ars2 or DGCR8 siRNA on microRNA expression
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Ars2 is a component of the nuclear cap-binding complex that is required for cellular proliferation and contributes to microRNA biogenesis. Arrays were performed to determine the repertoire of microRNAs that change following knock-down of Ars2. Knock-down of DGCR8 was also performed to determine which microRNAs were expressed and had half-lives short enough to significantly decrease over the duration of the experiment.
Overall design 9 samples were analyzed including: three biological replicates of control siRNA-transfected HeLa cells, HeLa cells transfected with three independent siRNAs targeting Ars2, or HeLa cells transfected with three independent siRNAs targeting DGCR8.
Contributor(s) Olejniczak SH, Gruber JJ, Dreyfuss G, Thompson CB
Citation(s) 22244333
Submission date Dec 22, 2011
Last update date May 02, 2017
Contact name Scott H Olejniczak
Phone 716-912-2723
Organization name Memorial Sloan Kettering Cancer Center
Street address 417 E68th St
City New York
State/province NY
ZIP/Postal code 10044
Country USA
Platforms (1)
GPL8786 [miRNA-1] Affymetrix Multispecies miRNA-1 Array
Samples (9)
GSM853219 HeLa ctl siRNA, biological replicate 1, miRNA
GSM853220 HeLa ctl siRNA, biological replicate 2, miRNA
GSM853221 HeLa ctl siRNA, biological replicate 3, miRNA
This SubSeries is part of SuperSeries:
GSE34681 Effects of Ars2 or DGCR8 siRNA on gene and microRNA expression
BioProject PRJNA156235

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34680_RAW.tar 1.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table

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