|
| Status |
Public on Feb 01, 2012 |
| Title |
Effects of Ars2 or DGCR8 siRNA on microRNA expression |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Ars2 is a component of the nuclear cap-binding complex that is required for cellular proliferation and contributes to microRNA biogenesis. Arrays were performed to determine the repertoire of microRNAs that change following knock-down of Ars2. Knock-down of DGCR8 was also performed to determine which microRNAs were expressed and had half-lives short enough to significantly decrease over the duration of the experiment.
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| |
|
| Overall design |
9 samples were analyzed including: three biological replicates of control siRNA-transfected HeLa cells, HeLa cells transfected with three independent siRNAs targeting Ars2, or HeLa cells transfected with three independent siRNAs targeting DGCR8.
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| |
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| Contributor(s) |
Olejniczak SH, Gruber JJ, Dreyfuss G, Thompson CB |
| Citation(s) |
22244333 |
| |
| Submission date |
Dec 22, 2011 |
| Last update date |
May 02, 2017 |
| Contact name |
Scott H Olejniczak |
| E-mail(s) |
olejnics@mskcc.org
|
| Phone |
716-912-2723
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Street address |
417 E68th St
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10044 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
|
| Samples (9)
|
| GSM853219 |
HeLa ctl siRNA, biological replicate 1, miRNA |
| GSM853220 |
HeLa ctl siRNA, biological replicate 2, miRNA |
| GSM853221 |
HeLa ctl siRNA, biological replicate 3, miRNA |
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| This SubSeries is part of SuperSeries: |
| GSE34681 |
Effects of Ars2 or DGCR8 siRNA on gene and microRNA expression |
|
| Relations |
| BioProject |
PRJNA156235 |
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