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Status |
Public on Jan 31, 2012 |
Title |
Genome features of “Dark-fly”, a Drosophila line reared long-term in a dark environment |
Organism |
Drosophila melanogaster |
Experiment type |
Genome variation profiling by array
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Summary |
To address the molecular mechanisms underlying environmental adaptation, we studied a Drosophila melanogaster line, termed Dark-fly, which has been maintained in constant dark conditions for 57 years (1400 generations).The structural gene copy number changes between the dark fly and its control were assessed by aCGH array. The comparison showed that hundreds of genes in the dark fly bear duplications or deletions relative to the control line.
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Overall design |
The copy number increase and decrease in the dark flies were determined by two-channel array hybridization with the control line. In addition to biological replicates, a Cy5-Cy3 dye swap was performed.Self-hybridization was also conducted to serve as a quality control.
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Contributor(s) |
Zhou J, Fuse N |
Citation(s) |
22432011 |
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Submission date |
Jan 30, 2012 |
Last update date |
Aug 14, 2018 |
Contact name |
Jun Zhou |
E-mail(s) |
jzhou@oeb.harvard.edu
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Phone |
617-496-5540
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Fax |
617-496-5854
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Organization name |
Harvard University
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Department |
Organismic and Evolutionary Biology
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Street address |
16 Divinity Ave. Rm2112
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City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02138 |
Country |
USA |
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Platforms (1) |
GPL6056 |
HartlLab Drosophilamelanogaster PCRproducts 22k |
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Samples (8)
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Relations |
BioProject |
PRJNA152577 |