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Series GSE35419 Query DataSets for GSE35419
Status Public on Jan 01, 2013
Title Sox9 mediated differential gene expression was assessed in prostaspheres derived from urogenital sinus epithelial cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Prostate cancer is one of the most common malignancies and the second leading cause of death from cancer in men. The molecular mechanisms driving prostate carcinogenesis are complex; with several lines of evidence suggesting that the re-expression of conserved developmental programs play a key role. Through conditional gene targeting and organ grafting, we describe conserved roles for the transcription factor Sox9 in the initiation of both prostate organogenesis and prostate carcinogenesis in murine models. Abrogation of Sox9 expression prior to the initiation of androgen signaling blocks the initiation of prostate development. Similarly, Sox9 deletion in two genetic models of prostate cancer (TRAMP and Hi-Myc) blocks cancer initiation. Expression profiling of Sox9-null prostate epithelial cells reveals that the role of Sox9 in the initiation of prostate development may relate to its regulation of multiple cytokeratins and/or calcium-related proteins. Due to its essential role in cancer initiation, manipulation of Sox9 targets in at-risk men may prove useful in the chemoprevention of prostate cancer.
Overall design Sox9 differential gene expression in prostaspheres derived from the urogenital sinus epithelium was assessed by tow-colors direct comparisons of labeled moieties. Hybridizations were performed on the Agilent (Santa Clara, CA) Whole Mouse Genome DNA microarray (mgug4122a). Sox9 targets were assessed in murine prostate epithelial cells with targeted deletion.
Contributor(s) Marchionni L, Huang Z, Simons B, Ross AE, Hurley P, Berman DM, Schaeffer EM
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Submission date Jan 30, 2012
Last update date May 10, 2018
Contact name Luigi Marchionni
Phone 410-502-8179
Organization name Johns Hopkins University
Department Oncology
Lab Cancer Biology Program
Street address 1550 Orleans St., CRB2, Room 1M52
City Baltimore
State/province MD
ZIP/Postal code 21231
Country USA
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (2)
GSM867885 No.2 vs No.7 Tamoxifen-treated wild type prostaspheres versus Tamoxifen-treated Sox9 prostaspheres
GSM867886 No.6 vs No.9 Vehicle-treated Sox9 prostaspheres versus Tamoxifen-treated Sox9 prostaspheres
BioProject PRJNA152579

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Supplementary file Size Download File type/resource
GSE35419_RAW.tar 37.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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