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| Status |
Public on Jul 19, 2012 |
| Title |
APJ1 and GRE3 Homologs Work in Concert to Allow Growth in Xylose in a Genetically Intractable Natural Saccharomyces sensu stricto Hybrid Yeast |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome variation profiling by array
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| Summary |
Creating Saccharomyces yeasts capable of efficient fermentation of pentoses such as xylose remains a key challenge in the production of ethanol from lignocellulosic biomass. Metabolic engineering of industrial Saccharomyces cerevisiae strains has yielded xylose-fermenting strains, but these strains have not yet achieved industrial viability due largely to xylose fermentation being prohibitively slower than that of glucose. Recently, it has been shown that naturally occurring xylose-utilizing Saccharomyces species exist. Uncovering the genetic architecture of such strains will shed further light on xylose metabolism, suggesting additional engineering approaches or possibly even the development of xylose-fermenting yeasts that are not genetically modified. We previously identified a hybrid yeast strain, the genome of which is largely Saccharomyces uvarum, which has the ability to grow on xylose as the sole carbon source. Despite the sterility of this hybrid strain, we were able to develop novel methods to genetically characterize its xylose utilization phenotype, using bulk segregant analysis in conjunction with high-throughput sequencing. We found that its growth in xylose is governed by at least two genetic loci: one of the loci maps to a known xylose-pathway gene, a novel allele of the aldo-keto reductase gene GRE3, while a second locus maps to an allele of APJ1, a chaperonin gene not previously connected to xylose metabolism. Our work demonstrates that the power of sequencing combined with bulk segregant analysis can also be applied to a non-genetically-tractable hybrid strain that contains a complex, polygenic trait, and it identifies new avenues for metabolic engineering as well as for construction of non-genetically modified xylose-fermenting strains.
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| Overall design |
comparative genomic hybridization by array
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| Contributor(s) |
Dunn B |
| Citation(s) |
22426884 |
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| Submission date |
Feb 03, 2012 |
| Last update date |
Jul 19, 2012 |
| Contact name |
Barbara Dunn |
| E-mail(s) |
bdunn@stanford.edu
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| Phone |
650-498-5995
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| Organization name |
Stanford University
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| Department |
Genetics
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| Street address |
-
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA152253 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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