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| Status |
Public on Jun 01, 2012 |
| Title |
Transcriptional profiling of turkey muscle satellite cells transfected with DAP-siRNA during proliferation and differentiation |
| Organism |
Meleagris gallopavo |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study, we directly compared turkey muscle satellite cell gene expression between satellite cells with the gene Death-Associated Protein (DAP) knocked down by siRNA transfection and those transfected with a lipofectamine control using our 6K Turkey Skeletal Muscle Long Oligo (TSKMLO) microarray (GPL9788). The DAP gene was previously identified as differentially expressed by genetic line and during development of turkey skeletal muscle (Sporer et al., 2011). Gene expression changes were investigated in satellite cells after 72 h of proliferation and after 48 h of differentiation. We identified novel candidate genes and pathways as playing potentially crucial roles in the DAP-mediated attenuation of the normal processes of proliferation and differentiation in turkey satellite cells previously identified by Velleman et al. (submitted).
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| Overall design |
This experiment was designed to investigate the role of DAP expression in turkey satellite cells during two crucial processes in muscle development: proliferation and differentiation. Satellite cells were isolated from 7-week-old turkeys from the RBC2 line. Cells were transfected with either DAP siRNA or a lipofectamine control; DAP expression was knocked down by over 50% with siRNA transfection (Velleman et al., submitted). Satellite cells were then induced to proliferate for 72h or differentiate for 48h; culture plates from each stage (n=4) were frozen until RNA extraction. Microarrays directly compared the DAP-knockdown to control from each of 4 culture plates and utilized a dye swap to equal 8 arrays for each of the cell developmental stages, proliferation and differentiation, and 16 arrays for the overall experiment investigating the role of DAP expression in satellite cell development and function. Hybridizations were performed in random order.
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| Contributor(s) |
Sporer KR, Velleman SG, Tempelman RJ, Strasburg GM |
| Citation(s) |
32922311 |
| |
| Submission date |
Feb 09, 2012 |
| Last update date |
Sep 21, 2020 |
| Contact name |
Kelly R Sporer |
| E-mail(s) |
buckhamk@msu.edu
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| Phone |
517-355-8474
|
| Fax |
517-432-0753
|
| Organization name |
Michigan State University
|
| Department |
Food Science and Human Nutrition
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| Lab |
Muscle Biology and Genetics Research
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| Street address |
3365 Anthony Hall
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| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
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| Platforms (1) |
| GPL9788 |
Turkey skeletal muscle long oligonucleotide (TSKMLO) microarray |
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| Samples (16)
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| GSM873168 |
DAP-knockdown vs Control (72h Proliferation) Replicate 1 |
| GSM873169 |
DAP-knockdown vs Control (72h Proliferation) Replicate 2 |
| GSM873170 |
DAP-knockdown vs Control (72h Proliferation) Replicate 3 |
| GSM873171 |
DAP-knockdown vs Control (72h Proliferation) Replicate 4 |
| GSM873172 |
DAP-knockdown vs Control (72h Proliferation) Replicate 5 |
| GSM873173 |
DAP-knockdown vs Control (72h Proliferation) Replicate 6 |
| GSM873174 |
DAP-knockdown vs Control (72h Proliferation) Replicate 7 |
| GSM873175 |
DAP-knockdown vs Control (72h Proliferation) Replicate 8 |
| GSM873176 |
DAP-knockdown vs Control (48h Differentiation) Replicate 1 |
| GSM873177 |
DAP-knockdown vs Control (48h Differentiation) Replicate 2 |
| GSM873178 |
DAP-knockdown vs Control (48h Differentiation) Replicate 3 |
| GSM873179 |
DAP-knockdown vs Control (48h Differentiation) Replicate 4 |
| GSM873180 |
DAP-knockdown vs Control (48h Differentiation) Replicate 5 |
| GSM873181 |
DAP-knockdown vs Control (48h Differentiation) Replicate 6 |
| GSM873182 |
DAP-knockdown vs Control (48h Differentiation) Replicate 7 |
| GSM873183 |
DAP-knockdown vs Control (48h Differentiation) Replicate 8 |
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| Relations |
| BioProject |
PRJNA152427 |
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