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Series GSE35836 Query DataSets for GSE35836
Status Public on Feb 16, 2012
Title Expression data from mouse neonatal ovarian 3MC culture experiments
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mammalian females are born with a finite number of non-renewing primordial follicles, the majority of which remain in a quiescent state for many years. Due to their non-renewing nature, these resting oocytes are particularly vulnerable to xenobiotic insult, resulting in premature ovarian senescence and the formation of dysfunctional oocytes. In this study we characterised the mechanisms of ovotoxicity for the ovotoxic agent 3-methylcholanthrene (3MC), which targets immature follicles. Neonatal mouse ovaries (PND3-4) were cultured in the presence of 3MC (5 µM) for 96 hours to observe their effects on the ovarian transcriptome. This was done in the hopes of gaining a better understanding of the mechanisms underpinning xenobiotic induced pre-antral ovotoxicity.
Overall design Ovaries from day 3-4 Swiss neonatal mice were cultured in 3MC containing media for 96 hours. The ovaries were then collected for RNA extraction and hybridization on an Illumina Sentrix Mouse ref-8 v2 Beadchip
Contributor(s) Sobinoff AP, McLaughlin EA
Citation(s) 22505044
Submission date Feb 15, 2012
Last update date Jun 14, 2018
Contact name Alexander Peter Sobinoff
Organization name University of Newcastle Australia
Department Science and IT
Lab Reproductive Science Group
Street address University Drive
City Newcastle
State/province NSW
ZIP/Postal code 2308
Country Australia
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM876018 Control (0.01% DMSO) rep 1
GSM876019 Control (0.01% DMSO) rep 2
GSM876020 Control (0.01% DMSO) rep 3
BioProject PRJNA151963

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35836_RAW.tar 3.1 Mb (http)(custom) TAR
GSE35836_Report-DMSO_vs_3MC.xlsx.gz 3.4 Mb (ftp)(http) XLSX
GSE35836_non_normalized.txt.gz 439.2 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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