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| Status |
Public on Feb 22, 2012 |
| Title |
Digital gene expression tag profiling of M. grisea G11 and T146 |
| Organism |
Pyricularia grisea |
| Experiment type |
Expression profiling by SAGE
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| Summary |
The rice blast fungus Magnaporthe oryzae is a major constraint to the world food security. To study the intracellular mechanism involved in its pathogenic morphogenesis, digital gene expression tag profiling was used to compare a previously generated temperature sensitive mutant, T146, which stopped pathogenic morphogenesis at 33°C, with its related wide type, Guy11. 416 genes were detected as differential expressed genes, 26 of which were specifically expressed in Guy11 but stopped expression in the mutant. Functional analysis revealed the mutated morphology maybe caused by a defection in translational or mitochondrial related processes. The results and the protocol used will improve our knowledge on the formation of the ts mutant and promote the further study on M. oryzae pathogenesis.
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| Overall design |
In our experiments, two samples are analyzed. There are no replicates included.
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| Contributor(s) |
hongkai W, fei X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 15, 2012 |
| Last update date |
Sep 28, 2016 |
| Contact name |
liu xiaoguang |
| E-mail(s) |
fengyuntwins2@163.com
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| Phone |
13819479661
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| Organization name |
Zhejiang University
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| Street address |
yuhang road
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| City |
hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platforms (1) |
| GPL15220 |
SAGE:17:NlaIII:Magnaporthe grisea |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA151849 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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