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Series GSE35882 Query DataSets for GSE35882
Status Public on Feb 21, 2012
Title Whole Cell Extract
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Most human genes are loaded with promoter proximally paused RNA polymerase II (RNAP II) molecules that are poised for release into productive elongation by P-TEFb. Gdown1, a protein that renders RNAP II responsive to mediator, is involved in RNAP II elongation control. During in vitro transcription assays Gdown1 specifically blocked elongation stimulation by TFIIF, inhibited the termination activity of TTF2, and influenced pausing factors NELF and DSIF, but did not affect the function of TFIIS or the mRNA capping enzyme. Without P-TEFb, Gdown1 led to the production of stably paused polymerases in the presence of nuclear extract. ChIP-Seq data demonstrated that Gdown1 mapped over essentially all poised polymerases across the human genome. Gdown1 increases the stability of poised polymerases while maintaining their responsiveness to P-TEFb, and mediator overcomes a Gdown1-mediated block of initiation by allowing TFIIF function.
Overall design 1 whole cell extract sample in human HeLa cell. See associated publication.
Contributor(s) Li T, Price DH
Citation(s) 22244331
Submission date Feb 16, 2012
Last update date May 15, 2019
Contact name Tiandao Li
Organization name Washington University
Street address 4444 Forest Park Ave
City St Louis
State/province MO
ZIP/Postal code 63108
Country USA
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (1)
GSM876989 RNA Pol II WCE
This SubSeries is part of SuperSeries:
GSE35886 Renormalization of GSE32442 and GSE33128 plus 1 control sample
SRA SRP010963
BioProject PRJNA155713

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35882_RAW.tar 49.1 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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