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| Status |
Public on Mar 26, 2012 |
| Title |
Prostaglandin D2 Inhibits Hair Growth and Is Elevated in Bald Scalp of Men with Androgenetic Alopecia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Testosterone is necessary for the development of male pattern baldness, known as androgenetic alopecia (AGA); yet the mechanisms for decreased hair growth in this disorder are unclear. Here, we show that prostaglandin D2 synthase (PTGDS) is elevated at the mRNA and protein levels in bald scalp compared to haired scalp of men with AGA. The product of PTGDS enzyme activity, prostaglandin D2 (PGD2), is similarly elevated in bald scalp. During normal follicle cycling in mice Ptgds and PGD2 levels increase immediately preceding the regression phase, suggesting an inhibitory effect on hair growth. We show that PGD2 inhibits hair growth in explanted human hair follicles and when applied topically to mice. Hair growth inhibition requires the PGD2 receptor G protein-coupled receptor 44 (GPR44), but not the prostaglandin D2 receptor 1(PTGDR). Furthermore, we find that a transgenic mouse, K14-Ptgs2, which targets prostaglandin-endoperoxide synthase 2 expression to the skin, demonstrates elevated levels of PGD2 in the skin and develops alopecia, follicular miniaturization and sebaceous gland hyperplasia, which are all hallmarks of human AGA. These results define PGD2 as an inhibitor of hair growth in AGA and suggest the PGD2-GPR44 pathway as a potential target for treatment.
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| Overall design |
5 individuals with Androgenetic Alopecia were biopsied at both their haired and bald scalp for mRNA purification and microarray (total 10 arrays)
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| |
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| Contributor(s) |
Garza LA, Cotsarelis G |
| Citation(s) |
22440736 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K08 AR055666 |
The role of prostaglandin d2 in hair follicle biology |
JOHNS HOPKINS UNIVERSITY |
Luis Andres Garza |
|
| |
| Submission date |
Feb 29, 2012 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Luis Andres Garza |
| E-mail(s) |
LAG@jhmi.edu
|
| Phone |
410-955-8662
|
| Organization name |
Johns Hopkins School of Medicine
|
| Department |
Dermatology
|
| Lab |
Garza lab
|
| Street address |
1550 Orleans St. Suite 204 CRBII Koch
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (10)
|
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| Relations |
| BioProject |
PRJNA152907 |
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