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Series GSE36256 Query DataSets for GSE36256
Status Public on Mar 06, 2012
Title Differential expression of miRNA in stretched rat alveolar epithelial cells
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Rattus norvegicus
Experiment type Non-coding RNA profiling by array
Summary Cyclic stretch of alveoli is characteristic of mechanical ventilation, and is postulated to be partly responsible for the lung injury and inflammation in ventilator induced lung injury. We propose that miRNAs may regulate some of the stretch response and, therefore, hypothesized that miRNAs would be differentially expressed between stretched and unstretched rat alveolar epithelial cells (RAECs).
Overall design RAECs were isolated and cultured to express type I epithelial characteristics, after which they were equibiaxially stretched to 25% change in surface area at 15 cycles/minute for 1 or 6 hrs, or served as unstretched controls, and miRNA were extracted (n = 4 for all groups).
Contributor(s) Yehya N, Yerrapureddy A, Tobias J, Margulies SS
Citation(s) 22537220
Submission date Mar 04, 2012
Last update date Jan 13, 2016
Contact name Nadir Yehya
Organization name University of Pennsylvania
Department Bioengineering
Street address 390 Towne Bldg, U Penn
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL7722 miRCURY LNA microRNA Array, v.10.0 - hsa, mmu & rno
Samples (12)
GSM885546 Stretch 1 hour sample 6
GSM885547 Stretch 6 hours sample 6
GSM885548 Control sample 6
BioProject PRJNA153075

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36256_RAW.tar 5.6 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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