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Series GSE36262 Query DataSets for GSE36262
Status Public on Mar 06, 2012
Title Array CGH of Drosophila ChrX deficiencies_duplications on heterochromatin custom array.
Organism Drosophila melanogaster
Experiment type Genome variation profiling by array
Summary Mapping the Drosophila melanogaster X heterochromatin by CGH analysis of embryos lacking specific regions of the X chromosome.
 
Overall design Eight ChrX deficiencies and duplications were tested: 168 (Dp(1;Y)y[2]67g), 175 (Dp(1;Y)y[2]sc), 114n (Dp(1;Y)y[+]mal[126]), 114f (Df(1)fog[114]), 186n (Dp(1;Y)y[+]mal), 186f (Df(1)mal12), B2580 (Dp(1;Y)ct y[+]), B5280 (Dp(1;Y)ct[+]y[+]). To generate embryos lacking different portions of the X heterochromatin, two types of stocks were used: (1) males carrying a deficient X chromosome missing part of the heterochromatin (generally fog-) and a duplication on the Y chromosome that complements the deficiency, and (2) males carrying a duplication of X on the Y chromosome which covers part of the X heterochromatin. In both cases males were crossed to attached X females. In the first case, one quarter of embryos lack most of the X chromosome except the duplication of X on Y. These embryos were identified according to their defects during cellularization. Another quarter of the embryos carry the deficient X chromosome as their sole X chromosome. These embryos were identified according to their defects in posterior midgut formation during early gastrulation. In the second case, one quarter of embryos that lack most of the X chromosome except the duplication of X on Y were identified according to their defects in cellularization or posterior midgut formation. All embryos were collected at room temperature.
 
Citation(s) 22745230
Submission date Mar 05, 2012
Last update date Jan 18, 2013
Contact name Bing He
E-mail(s) bhe@princeton.edu
Organization name Princeton University
Department Molecular Biology
Lab Eric Wieschaus
Street address Washington Rd
City Princeton
State/province NJ
ZIP/Postal code 08544
Country USA
 
Platforms (1)
GPL15321 Agilent-024708: Drosophila euchromatic probes, plus potential heterochromatin [Probe name version]
Samples (8)
GSM885613 168: Xhet mapping
GSM885614 175: Xhet mapping
GSM885615 114n: Xhet mapping
Relations
BioProject PRJNA153087

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36262_RAW.tar 257.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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