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Series GSE3628 Query DataSets for GSE3628
Status Public on Jan 01, 2006
Title Rat Normal Cochlear Nucleus
Organism Rattus norvegicus
Experiment type Expression profiling by SAGE
Summary The cochlear nucleus is the first central pathway involved in the processing of peripheral auditory activity. It is heterogeneous in neuronal populations and physiologic responses and is organized in three major subdivisions: the anterior ventral cochlear nucleus (AVCN), the posterior ventral cochlear nucleus (PVCN) and the dorsal cochlear nucleus (DCN). Although each region demonstrates multiple cell types and functions, there are predominant populations of neurons in each region that underlie the principal role each subdivision plays in auditory processing. Little is known of the underlying genetic contribution to these properties. This study sought to identify genes expressed in the subdivisions of the cochlear nucleus that may account for the anatomical and physiological characteristics of each subdivision. These data provide a genetic basis for understanding normal auditory processing in the cochlear nucleus and a template for investigating changes that may occur with hearing loss, the generation and percept of tinnitus, and central processing disorders.
Keywords: normal, comparative
Overall design Brown Norway rats (n=40, female, 45days) were anesthetized and decapitated. Brains were rapidly removed and the subdivisions of the cochlear nucleus (AVCN, PVCN and DCN) dissected on dry ice. Total RNA was extracted and tested for concentration and purity by spectrophotometry and integrity by gel electrophoresis. SAGE was performed using the NlaIII enzyme and Invitrogen SAGE kit. Concatemers were commercially sequenced and imported into eSAGE (Margulies and Innis, 2000) for tag extraction and frequency.
Contributor(s) Friedland DR, Eernisse R, Popper P, Cioffi JA
Citation(s) 16905270, 16550590
Submission date Nov 17, 2005
Last update date Mar 16, 2012
Contact name David R Friedland
Phone 414-805-5625
Fax 414-805-7936
Organization name Medical College of Wisconsin
Department Otolaryngology and Communication Sciences
Street address 8701 Watertown Plank Rd
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
Platforms (1)
GPL23 SAGE:10:NlaIII:Rattus norvegicus
Samples (3)
GSM83999 Rat_Normal_AVCN
GSM84000 Rat_Normal_PVCN
GSM84001 Rat_Normal_DCN
BioProject PRJNA93737

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