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Series GSE3642 Query DataSets for GSE3642
Status Public on Feb 01, 2006
Title Single-cell aneuploidy detection by array CGH
Organism Homo sapiens
Experiment type Genome variation profiling by genome tiling array
Summary In this study, we extend array CGH technology by making the accurate detection of chromosomal imbalances possible from a single fibroblast and blastomere following Phi29 DNA polymerase amplification.
Keywords: CGH
Overall design Firstly, array CGH experiments were performed on four different fibroblast cell lines, derived from patients affected by, respectively, trisomy 13, 18, 21, and monosomy X. For each cell line, three single cells were amplified. Following DNA amplification, all cells showed the expected DNA yields (n=12; 1.87 µg plus:minus 0.39). Sex-mismatch array CGH experiments were conducted on amplified DNA samples obtained from each cell. Sex chromosome ploidy levels, as well as all expected autosomal abnormalities were clearly identified. Secondly, we applied single-cell aneuploidy screening for the detection of chromosomal imbalances in preimplantation embryos. DNA from blastomeres from three 7-8 cell-stage embryos was amplified by Phi29 DNA polymerase. Following DNA amplification, all cells yielded the expected amount of DNA (n=16; 2.45 µg plus:minus 0.41). Chromosomal aneuploidies were accurately detected using a simple and rapid array CGH protocol.
Contributor(s) Vermeesch JR, Le Caignec C, Thienpont B, Moreau Y, Spits C, Sermon K, De Rycke M, Fryns J, Liebaers I, Van Steirteghem A
Citation(s) 16698960
Submission date Nov 21, 2005
Last update date Apr 17, 2012
Contact name Joris R Vermeesch
Organization name Katholic University Leuven
Department Center for Human Genetics
Street address Herestraat, 49
City Leuven
ZIP/Postal code 3000
Country Belgium
Platforms (1)
GPL3091 KUL Human 4k BAC array Aneuploidy Screening
Samples (29)
GSM84231 CGH_2005051140_T13_cell1
GSM84244 CGH_2005051141_T13_cell2
GSM84247 CGH_2005051142_T13_cell3
BioProject PRJNA93765

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