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Series GSE3642

Query DataSets for GSE3642

Status

Public on Feb 01, 2006

Title

Single-cell aneuploidy detection by array CGH

Organism

Homo sapiens

Experiment type

Genome variation profiling by genome tiling array

Summary

In this study, we extend array CGH technology by making the accurate detection of chromosomal imbalances possible from a single fibroblast and blastomere following Phi29 DNA polymerase amplification.
Keywords: CGH

Overall design

Firstly, array CGH experiments were performed on four different fibroblast cell lines, derived from patients affected by, respectively, trisomy 13, 18, 21, and monosomy X. For each cell line, three single cells were amplified. Following DNA amplification, all cells showed the expected DNA yields (n=12; 1.87 µg plus:minus 0.39). Sex-mismatch array CGH experiments were conducted on amplified DNA samples obtained from each cell. Sex chromosome ploidy levels, as well as all expected autosomal abnormalities were clearly identified. Secondly, we applied single-cell aneuploidy screening for the detection of chromosomal imbalances in preimplantation embryos. DNA from blastomeres from three 7-8 cell-stage embryos was amplified by Phi29 DNA polymerase. Following DNA amplification, all cells yielded the expected amount of DNA (n=16; 2.45 µg plus:minus 0.41). Chromosomal aneuploidies were accurately detected using a simple and rapid array CGH protocol.

Contributor(s)

Vermeesch JR, Le Caignec C, Thienpont B, Moreau Y, Spits C, Sermon K, De Rycke M, Fryns J, Liebaers I, Van Steirteghem A

Citation(s)

16698960

Submission date

Nov 21, 2005

Last update date

Apr 17, 2012

Contact name

Joris R Vermeesch

E-mail(s)

Joris.Vermeesch@uz.kuleuven.ac.be