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| Status |
Public on Apr 26, 2012 |
| Title |
The RDE-10/RDE-11 complex triggers RNAi induced mRNA degradation by association with target mRNA in C. elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
The molecular mechanisms for target mRNA degradation in C. elegans undergoing RNA interference (RNAi) are not fully understood. Using a combination of genetic, proteomic and biochemical approaches, we report a divergent RDE-10/RDE-11 complex that is required for RNAi in C. elegans. The RDE-10/RDE-11 complex acts in parallel of nuclear RNAi. Association of the complex with target mRNA is dependent on RDE-1 but not RRF-1, suggesting that target mRNA recognition depends on primary but not secondary siRNA. Furthermore, RDE-11 is required for mRNA degradation subsequent to target engagement. Deep sequencing reveals a 5-fold decrease in secondary siRNA abundance in rde-10 and rde-11 mutant animals, while primary siRNA and micro-RNA biogenesis is normal. Therefore, the RDE-10/RDE-11 complex is critical for amplifying the exogenous RNAi response. Our work uncovers an essential output of the RNAi pathway in C. elegans.
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| Overall design |
21-24nt small RNA were purifed from different C. elegans strain populations that underwent sel-1 RNAi
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| Contributor(s) |
Yang H, Vallandingham J, Li H, Mak HY |
| Citation(s) |
22508728 |
| |
| Submission date |
Mar 14, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Huan Yang |
| Organization name |
Stowers Institute for Medical Research
|
| Lab |
Mak Lab
|
| Street address |
1000 East 50th Street
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (12)
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| Relations |
| SRA |
SRP011475 |
| BioProject |
PRJNA153535 |
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