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Series GSE36704 Query DataSets for GSE36704
Status Public on Jun 12, 2017
Title Identification of microRNAs in response to blue light irridiation by high-throughput sequencing in Saccharina japonica
Organism Saccharina japonica
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Blue light (BL) is an important environmental factor that plays critical role in algae growth and development. Saccharina japonica, as a typical brown alga, showed greatly affected by BL. However, little has been known about the regulation pathway of BL response in algae. microRNAs (miRNAs) participated in great number of life process regulation and may be also involved in the BL response in plants. To identify miRNAs from S. japonica and characterize their probable roles in BL response, we sequenced and compared small RNA libraries under BL irradiation and dark conditions. 20 potential novel miRNAs were identified from S. japonica. Bioinformatics analysis of the miRNAs indicated that their potential targets were involved in various biological processes. Based on differential expression analysis and qRT-PCR experiment, some probable miRNAs related to BL responses were selected for further verification of their function, such as miR398. Our results demonstrated that miRNAs might play vital roles in metabolism of S. japonica, including BL responses.
Overall design microRNA profiling of two samples treated by blue light (BL) and dark (DK) by high-throughput sequencing
Contributor(s) Li W, Duan D
Citation(s) 28338896
Submission date Mar 22, 2012
Last update date May 15, 2019
Contact name Wei Li
Organization name Institute of Oceanology, Chinese Academy of Sciences
Street address No 7 Nanhai Road
City Qingdao
State/province Shandong
ZIP/Postal code 266071
Country China
Platforms (1)
GPL14911 Illumina HiSeq 2000 (Saccharina japonica)
Samples (2)
GSM899269 sj-BL
GSM899270 sj-DK
BioProject PRJNA153567
SRA SRP011900

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Supplementary file Size Download File type/resource
GSE36704_differentially_expressed_known_miRNAs.txt.gz 20.9 Kb (ftp)(http) TXT
GSE36704_known_miRNAs_in_BL_and_DK_samples.txt.gz 29.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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