|
| Status |
Public on Feb 28, 2013 |
| Title |
A role for the nucleoporin Nup170p in chromatin structure and gene silencing |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Genome binding/occupancy profiling by array
Expression profiling by array
|
| Summary |
Embedded in the nuclear envelope, nuclear pore complexes (NPCs) not only regulate nuclear transport, but also interface with both transcriptionally active euchromatin and largely silenced heterochromatin, as well as the boundaries between these regions. It is unclear what functional role NPCs play in establishing or maintaining these distinct chromatin domains. Here we report that the yeast NPC protein Nup170p interacts with specific regions of the genome containing ribosomal protein and subtelomeric genes. At these locations, Nup170p functions to establish normal nucleosome positioning and as a repressor of transcription. We show that the function of Nup170p in subtelomeric gene silencing is linked to its association with the RSC chromatin-remodeling complex and the silencing factor Sir4p, and that the binding of Nup170p and Sir4p to subtelomeric chromatin is cooperative and necessary for the association of telomeres with the nuclear envelope. Our results establish the NPC as an active participant in the formation of peripheral heterochromatin.
This SuperSeries is composed of the SubSeries listed below.
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| |
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| Overall design |
Refer to individual Series
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| |
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| Citation(s) |
23452847 |
| |
| Submission date |
Mar 26, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Yakun Wan |
| E-mail(s) |
ywansystemsbiology@gmail.com
|
| Organization name |
Southeast University
|
| Street address |
Sipailou #2
|
| City |
Nanjing |
| ZIP/Postal code |
210096 |
| Country |
China |
| |
|
| Platforms (3) |
| GPL4131 |
Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A) |
| GPL6068 |
Agilent-011447 Yeast Oligo Microarray (G4140A) - alternative version |
| GPL9377 |
Illumina Genome Analyzer II (Saccharomyces cerevisiae) |
|
| Samples (14)
|
| GSM901163 |
Genome-wide nucleosome positioning in wild type cells |
| GSM901164 |
Genome-wide nucleosome positioning in nup170∆ cells |
| GSM901165 |
nup157∆ gene expression analysis |
| GSM901166 |
nup170∆ gene expression analysis |
| GSM901167 |
nup188∆ gene expression analysis |
| GSM901168 |
Sth1p depletion 2hr gene expression analysis |
| GSM901169 |
Sth1p reinduction 4hr gene expression analysis |
| GSM901170 |
Gene expression analysis following methionine addition to wild type cells |
| GSM901171 |
Nup157-myc ChIP from wild type cells |
| GSM901172 |
Nup170-myc ChIP from wild type cells |
| GSM901173 |
Nup170-myc ChIP from sir4∆ cells |
| GSM901174 |
Nup170-myc ChIP from yku70∆ cells |
| GSM984791 |
Genome-wide nucleosome positioning in sth1p_depletion_0h cells |
| GSM984792 |
Genome-wide nucleosome positioning in sth1p_depletion_2h cells |
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| This SuperSeries is composed of the following SubSeries: |
| GSE36792 |
The nucleoporin Nup170p is required for subtelomeric chromatin structure and gene silencing. [MNase-Seq] |
| GSE36793 |
A role for the nucleoporin Nup170p in chromatin structure and gene silencing [Agilent microarrays] |
| GSE36794 |
A role for the nucleoporin Nup170p in chromatin structure and gene silencing [Agilent ChIP-chip] |
|
| Relations |
| BioProject |
PRJNA157329 |
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