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Series GSE36827 Query DataSets for GSE36827
Status Public on Mar 25, 2014
Title HDAC7 is a repressor of myeloid genes whose downregulation in pre-B cells is required for transdifferentiation into macrophages
Organism Mus musculus
Experiment type Expression profiling by array
Summary In the immune system HDAC7 is expressed in T cells where it regulates the expression of key genes for T cell development and function. Here we report that HDAC7 is also highly expressed in B cell precursors, where it is recruited by MEF2C to repress the activity of key genes for myeloid cell function. While HDAC7 is down-regulated during the conversion of pre-B cells into macrophages, re-expression of HDAC7 interferes with both the acquisition of the myeloid gene transcriptional program and macrophage specific cell functions. Thus, HDAC7 is a novel transcriptional repressor of lineage inappropriate genes in B cells.
Overall design Biological duplicates of C10-MSCV and C10-HDAC7 cells were un-iduced or induced to transdifferentiate for 48 and 72 hors. Total RNA from cultured cells was extracted by Trizol and then purified. PCR amplified RNAs were hybridized against Affymetrix mouse arrays chip (Mouse Genome 430 PM strip).
Contributor(s) Parra M, Islam AB
Citation(s) 23696748
Submission date Mar 27, 2012
Last update date Aug 06, 2018
Contact name Maribel Parra
E-mail(s), khademul.islam@gmailcom
Phone +34932607133
Fax +34932607219
Organization name Bellvitge Biomedical Research Institute (IDIBELL)
Department Cancer Epigenetics and Biology Programme (PEBC)
Lab Cellular Differentiation
Street address Avda. Gran Via 199-203, L'Hospitalet de Llobregat
City Barcelona
State/province Barcelona
ZIP/Postal code 08908
Country Spain
Platforms (1)
GPL11180 [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate
Samples (12)
GSM902524 C10-MSCV cells untreated, biological rep1
GSM902525 C10-MSCV cells untreated, biological rep2
GSM902526 C10-MSCV cells treated with β-estradiol for 48h, biological rep1
BioProject PRJNA156927

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Supplementary file Size Download File type/resource
GSE36827_RAW.tar 21.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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