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Series GSE36965 Query DataSets for GSE36965
Status Public on Mar 24, 2014
Title ChIP-chip analysis of GBF1 in the presence and absence of its heterodimerizing partners, HY5 and HYH
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary Transcription factors are key components of light signalling as they amplify the signal, which results in massive changes in genome-wide expression during photomorphogenesis. Three bZIP transcription factors (TFs), namely GBF1, HY5, and HYH, form heterodimers with each other and regulate photomorphogenesis in an interdependent manner. GBF1 acts as both a positive and negative regulator of photomorphogenesis, whereas HY5 and HYH mainly act as positive regulators of photomorphogenesis. In this study, the impact of heterodimerization of GBF1 with HY5 and HYH was analyzed for genome-wide binding of GBF1 through ChIP-chip in GBF1. We identified more than 2000 direct targets of GBF1 in the presence of HY5 and HYH. However, in the absence of HY5, very few binding sites were found, and in the absence of functional HYH protein, the number of GBF1 direct targets reduced to only half compared to when functional HYH was present.
Overall design ChIPed DNA with GBF1 antibody from GBF1OE, hy5 GBF1OE, and hyh GBF1OE lines vs. ChIPed DNA with GBF1 antibody from wild-type.
Contributor(s) Ram H, Jain M, Chattopadhyay S
Citation(s) 24157608
Submission date Mar 30, 2012
Last update date May 09, 2017
Contact name Sudip Chattopadhyay
Organization name National Institute of Plant Genome Research
Department Development Biology
Lab 101
Street address Aruna Asaf Ali Marg, JNU Campus
City New Delhi
State/province Delhi
ZIP/Postal code 110067
Country India
Platforms (1)
GPL10977 [At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array
Samples (3)
GSM907510 GBF1OE
GSM907511 hy5GBF1OE
GSM907512 hyhGBF1OE
This SubSeries is part of SuperSeries:
GSE49337 Heterodimerization of GBF1 with HY5 and HYH is crucial for its genome wide binding and target gene regulation
BioProject PRJNA157299

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36965_RAW.tar 571.0 Mb (http)(custom) TAR (of BAR, BED, CEL)
Processed data provided as supplementary file

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