 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 24, 2014 |
| Title |
ChIP-chip analysis of GBF1 in the presence and absence of its heterodimerizing partners, HY5 and HYH |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Transcription factors are key components of light signalling as they amplify the signal, which results in massive changes in genome-wide expression during photomorphogenesis. Three bZIP transcription factors (TFs), namely GBF1, HY5, and HYH, form heterodimers with each other and regulate photomorphogenesis in an interdependent manner. GBF1 acts as both a positive and negative regulator of photomorphogenesis, whereas HY5 and HYH mainly act as positive regulators of photomorphogenesis. In this study, the impact of heterodimerization of GBF1 with HY5 and HYH was analyzed for genome-wide binding of GBF1 through ChIP-chip in GBF1. We identified more than 2000 direct targets of GBF1 in the presence of HY5 and HYH. However, in the absence of HY5, very few binding sites were found, and in the absence of functional HYH protein, the number of GBF1 direct targets reduced to only half compared to when functional HYH was present.
|
| |
|
| Overall design |
ChIPed DNA with GBF1 antibody from GBF1OE, hy5 GBF1OE, and hyh GBF1OE lines vs. ChIPed DNA with GBF1 antibody from wild-type.
|
| |
|
| Contributor(s) |
Ram H, Jain M, Chattopadhyay S |
| Citation(s) |
24157608 |
| |
| Submission date |
Mar 30, 2012 |
| Last update date |
May 09, 2017 |
| Contact name |
Sudip Chattopadhyay |
| E-mail(s) |
sudipchatto@gmail.com
|
| Organization name |
National Institute of Plant Genome Research
|
| Department |
Development Biology
|
| Lab |
101
|
| Street address |
Aruna Asaf Ali Marg, JNU Campus
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110067 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL10977 |
[At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array |
|
| Samples (3) |
|
| This SubSeries is part of SuperSeries: |
| GSE49337 |
Heterodimerization of GBF1 with HY5 and HYH is crucial for its genome wide binding and target gene regulation |
|
| Relations |
| BioProject |
PRJNA157299 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE36965_RAW.tar |
571.0 Mb |
(http)(custom) |
TAR (of BAR, BED, CEL) |
| Processed data provided as supplementary file |
|
|
|
|
 |
