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| Status |
Public on Nov 26, 2012 |
| Title |
Expression data from control and colicin M treated E. coli MG1655 culture |
| Platform organism |
Escherichia coli |
| Sample organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by array
|
| Summary |
Among colicin producing E. coli, colicin M producing strains are the most frequently present in natural populations. Bacteria must be able to sense and respond to unfavourable conditions, resulting in adaptive responses. To gain insight into colicin M ecological role and the purposes related to antimicrobial therapy, the effects of subinhibitory concentrations of colicin M on E. coli whole genome transcription was investigated. We used microarray analysis to follow differential gene expression in E. coli upon colicin M exposure. Colicin M inhibits peptidoglycan synthesis altering expression of genes involved in envelope stress, osmotic and other stresses, exopolysaccharide prodoction, biofilm formation, and cell motility.
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| Overall design |
A sub-lethal concentration of colicin M was used to treat E. coli MG1655 cultures at an early logarithmic phase. The experiments were performed in parallel with untreated control E. coli MG1655 cultures. Total RNA was extracted after 30 min and 60 min treatment with colicin M and used for hybridization on Affymetrix microarrays.
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| Contributor(s) |
Žgur-Bertok D, Kamenšek S |
| Citation(s) |
23421615 |
| |
| Submission date |
Apr 04, 2012 |
| Last update date |
Mar 08, 2019 |
| Contact name |
simona kamensek |
| E-mail(s) |
simona.kamensek@bf.uni-lj.si
|
| Organization name |
university of ljubljana, biotechnical faculty
|
| Department |
department of biology
|
| Street address |
večna pot 111
|
| City |
ljubljana |
| ZIP/Postal code |
1000 |
| Country |
Slovenia |
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| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
|
| Samples (8)
|
| GSM909095 |
control sample at 30 min, biological rep1 |
| GSM909096 |
treated sample at 30 min, biological rep1 |
| GSM909097 |
control sample at 60 min, biological rep1 |
| GSM909098 |
treated sample at 60 min, biological rep1 |
| GSM909099 |
control sample at 30 min, biological rep2 |
| GSM909100 |
treated sample at 30 min, biological rep2 |
| GSM909101 |
control sample at 60 min, biological rep2 |
| GSM909102 |
treated sample at 60 min, biological rep2 |
|
| Relations |
| BioProject |
PRJNA157845 |
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