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Series GSE37051 Query DataSets for GSE37051
Status Public on Sep 19, 2012
Title Identification of a FOXO3/IRF7 circuit that limits inflammatory sequelae of antiviral responses (expression)
Organism Mus musculus
Experiment type Expression profiling by array
Summary We have used an unbiased systems approach to predict that a member of the forkhead family of transcription factors, FOXO3, is a negative regulator of a subset of antiviral genes. This prediction was validated using macrophages isolated from Foxo3-null mice. We detected significantly increased transcription of a subset of interferon-stimulated genes (ISGs) under basal conditions in Foxo3-null macrophages when compared to their wild type (WT) counterparts, suggesting that FOXO3 functions as a repressor of these genes. Stimulation of Foxo3-null macrophages with poly-IC (PIC) further increased the levels of this subset of ISGs, and also revealed the transcription of additional ISGs.
 
Overall design C57BL/6 mice were obtained from Jackson Laboratories. Foxo3-/- mice in the FVB background were obtained from MMRRC and were backcrossed to C57BL/6 mice at least 5 times to generate congenic mice. C57BL/6 Foxo3+/- heterozygotes were intercrossed to generate Foxo3-/- mice. Mice were maintained at the animal facility of the Institute for Systems Biology and used at 8-12 weeks of age. All animals were housed and handled according to the approved protocols of the University of Washington and Institute for Systems Biology's Institutional Animal Care and Use Committees.

Bone marrow macrophages from wildtype and Foxo3 knock-out mice were stimulated with PIC or left untreated. 3 replicates per group.
 
Contributor(s) Raman A, Litvak V
Citation(s) 22982991
Submission date Apr 04, 2012
Last update date Sep 21, 2012
Contact name Ayush Raman
E-mail(s) ayush.raman@systemsbiology.org
Phone 206-732-1473
Organization name Institute for Systems Biology
Department Computational Dept.
Lab Shmulevich Lab
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
 
Platforms (1)
GPL15408 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [Alternative CDF: MBNI Entrez Gene version 11]
Samples (12)
GSM909340 Wildtype BMM Unstimulated 1
GSM909341 Wildtype BMM Unstimulated 2
GSM909342 Wildtype BMM Unstimulated 3
This SubSeries is part of SuperSeries:
GSE37052 Identification of a FOXO3/IRF7 circuit that limits inflammatory sequelae of antiviral responses
Relations
BioProject PRJNA157893

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37051_RAW.tar 377.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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