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Series GSE37545 Query DataSets for GSE37545
Status Public on Jul 02, 2012
Title Adaptive mutations that prevent cross-talk enable the expansion of paralogous signaling protein families
Organism Caulobacter vibrioides NA1000
Experiment type Expression profiling by array
Summary Orthologous proteins often harbor numerous substitutions, but whether these differences result from neutral or adaptive processes is usually unclear. To tackle this challenge, we examined the divergent evolution of a model bacterial signaling pathway comprising the kinase PhoR and its cognate substrate PhoB. We show that the specificity-determining residues of these proteins are typically under purifying selection, but have, in α-proteobacteria, undergone a burst of diversification followed by extended stasis. By reversing mutations that accumulated in an α-proteobacterial PhoR, we demonstrate that these substitutions were adaptive, enabling PhoR to avoid cross-talk with a paralogous pathway that arose specifically in α-proteobacteria. Our findings demonstrate that duplication and the subsequent need to avoid cross-talk strongly influence signaling protein evolution. These results provide a concrete example of how system-wide insulation can be achieved post-duplication through a surprisingly limited number of mutations. Our work may help explain the apparent ease with which paralogous protein families expanded in all organisms.
 
Overall design Two-condition experiment, mutant vs. WT in given growth media
 
Contributor(s) Capra EJ, Perchuk BS, Skerker JM, Laub MT
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Submission date Apr 24, 2012
Last update date Jul 04, 2012
Contact name Emily J Capra
E-mail(s) ecapra@mit.edu
Organization name Massachusetts Institute of Technology
Department Biology
Lab Laub
Street address 31 Ames Street, 68-570
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platforms (1)
GPL15487 Agilent-032577 Custom Caulobacter crescentus NA1000 15k array
Samples (7)
GSM921430 PhoR(TV) M2G vs. WT M2G
GSM921431 DPhoR M2G vs. WT-M2G
GSM921432 DPhoR M5G vs. WT-M5G
Relations
BioProject PRJNA161859

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37545_RAW.tar 10.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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