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| Status |
Public on Apr 30, 2012 |
| Title |
The effect of alpha, beta, gamma interferon receptors on host reponse to influenza virus in mouse embryonic fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The objective of this study was to examine the host transcriptional response to high and low pathogenecity viruses in mouse embryonic fibroblasts (MEFs) isolated from different mouse genetic backgrounds to further understand the contribution of interferon signaling pathways to host response to influenza.
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| Overall design |
WT, IFNgR-/-, IFNabR-/-, or IFNabgR-/- MEFs were mock-infected or infected with the A/WSN/33 (WSN), reconstructed 1918 (r1918), or A/Vietnam/1203/2004 (VN1203) strains of influenza virus at an MOI of 2 PFU/cell. At 24 h p.i., total RNA was isolated from the cells and used for microarray hybridization. Experiments with VN1203 and r1918 were performed seperately (experiment a) from experiments with WSN (experiment b). Appropriate mock samples were harvested in each experiment. All gene expression profiles are compared to time-, experiment-, and genotype-matched mock-infected samples.
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| Contributor(s) |
Goodman AG, Zeng H, Proll SC, Peng X, Cilloniz C, Carter VS, Korth MJ, Tumpey TM, Katze MG |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 30, 2012 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Michael Katze |
| E-mail(s) |
data@viromics.washington.edu
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| Organization name |
University of Washington
|
| Department |
Microbiology
|
| Lab |
Michael G. Katze, Ph.D
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| Street address |
Rosen Building 960 Republican St.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-4325 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA162581 |
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