NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE37779 Query DataSets for GSE37779
Status Public on Apr 27, 2013
Title The effect of HO-1 overexpression on microRNA transcriptome in NCI-H292 lung cancer cells
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The aim was to determine the effect of heme oxygenase-1 (HO-1) overexpression on microRNA transcriptome in human non-small cell lung carcinoma cell line (NCI-H292).
Since the cells of different HO-1 genotypes were used (cells are after retroviral transduction with empty vector with normal level of HO-1 or retroviral transduction with vector harboring HO-1), it is possible get the comprehensive answer which microRNAs are regulated by HO-1.
 
Overall design Confluent NCI-H292 cells that contain EV-ctrl or overexpressing HO-1. Pool of reference cells are cells untreated and treated with 10 ng/ml TNF-α for 6 hours before RNA isolation. The samples are biological triplicates - three independent experiments were performed at different time points for all cell lines. Total number of the presented samples is 6.
 
Contributor(s) Skrzypek K, Dulak J, Kieda C
Citation(s) 23617628
Submission date May 04, 2012
Last update date Sep 08, 2014
Contact name Klaudia Skrzypek
E-mail(s) klaudia.skrzypek@uj.edu.pl
Organization name Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
Department Department of Medical Biotechnology
Street address ul. Gronostajowa 7
City Krakow
ZIP/Postal code 30-387
Country Poland
 
Platforms (1)
GPL15532 miRCURY LNA microRNA Array, v.11.0 covering miRBASE version 14.0 at the Sanger Institute
Samples (6)
GSM928159 NCI-H292 EV-ctrl replicate 1
GSM928160 NCI-H292 HO-1 replicate 1
GSM928161 NCI-H292 EV-ctrl replicate 2
Relations
BioProject PRJNA163263

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37779_RAW.tar 9.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap