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Series GSE37779 Query DataSets for GSE37779
Status Public on Apr 27, 2013
Title The effect of HO-1 overexpression on microRNA transcriptome in NCI-H292 lung cancer cells
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The aim was to determine the effect of heme oxygenase-1 (HO-1) overexpression on microRNA transcriptome in human non-small cell lung carcinoma cell line (NCI-H292).
Since the cells of different HO-1 genotypes were used (cells are after retroviral transduction with empty vector with normal level of HO-1 or retroviral transduction with vector harboring HO-1), it is possible get the comprehensive answer which microRNAs are regulated by HO-1.
Overall design Confluent NCI-H292 cells that contain EV-ctrl or overexpressing HO-1. Pool of reference cells are cells untreated and treated with 10 ng/ml TNF-α for 6 hours before RNA isolation. The samples are biological triplicates - three independent experiments were performed at different time points for all cell lines. Total number of the presented samples is 6.
Contributor(s) Skrzypek K, Dulak J, Kieda C
Citation(s) 23617628
Submission date May 04, 2012
Last update date Sep 08, 2014
Contact name Klaudia Skrzypek
Organization name Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
Department Department of Medical Biotechnology
Street address ul. Gronostajowa 7
City Krakow
ZIP/Postal code 30-387
Country Poland
Platforms (1)
GPL15532 miRCURY LNA microRNA Array, v.11.0 covering miRBASE version 14.0 at the Sanger Institute
Samples (6)
GSM928159 NCI-H292 EV-ctrl replicate 1
GSM928160 NCI-H292 HO-1 replicate 1
GSM928161 NCI-H292 EV-ctrl replicate 2
BioProject PRJNA163263

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE37779_RAW.tar 9.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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