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Series GSE38213 Query DataSets for GSE38213
Status Public on May 25, 2012
Title Identification and comparative analysis of microRNAs associated with low-N tolerance in rice genotypes
Platform organism synthetic construct
Sample organism Oryza sativa
Experiment type Non-coding RNA profiling by array
Summary Micro-RNA associated with low-N tolerance have been identified in rice (Oryza sativa) genotypes on the basis of differential expression of miRNA in low-N tolerant and low-N sensitive genotypes of rice. Since, miRNAs emerged as key players in plant responses and adaptation to stress, identification of low-N responsive miRNAs and their targets could help to uncover the molecular mechanisms of N-deficiency tolerance in plants.
We used affymetrix based GeneChip miRNA_2.0 Array to detail the global programme of miRNA expression variation in low-N tolerant (IC-547557) and low -N sensitive (Vivek Dhan).
Overall design Low-N tolerant and low-N sensitive rice genotypes were grown using the nutrient medium containing 2 mM K2SO4, 2 mM MgSO4, 1 mM CaCl2, 0.3 mM NaH2PO4, 40 µM Fe-EDTA, 9 µM MnCl2, 25 µM Na2MoO4, 20 µM H3BO3, 1.5 µM ZnSO4, and 1.5 µM CuSO4. The nitrogen in the form of NH4NO3 was maintained as 0.01 mM (low-N condition). The pH of the nutrient solutions was adjusted to 6.0 and the solutions were changed every three days. Twenty-day-old plants were sampled for expression studies of miRNA.
Contributor(s) Ahmad A, Nischal L
Citation(s) 23227161
Submission date May 24, 2012
Last update date May 02, 2017
Contact name ALTAF AHMAD
Phone 911126059688
Fax 91112609663
Organization name Jamia Hamdard (Hamdard University)
Department Department of Botany
Lab Molecular Ecology Laboratory
Street address Faculty of Science
City New Delhi
State/province Delhi
ZIP/Postal code 110062
Country India
Platforms (1)
GPL14613 [miRNA-2] Affymetrix Multispecies miRNA-2 Array
Samples (4)
GSM936813 Vivek Dhan Biological replicate 1
GSM936814 Vivek Dhan Biological replicate 2
GSM936815 IC-547557 Biological replicate 1
BioProject PRJNA167508

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38213_RAW.tar 2.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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