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Series GSE38213 Query DataSets for GSE38213
Status Public on May 25, 2012
Title Identification and comparative analysis of microRNAs associated with low-N tolerance in rice genotypes
Platform organism synthetic construct
Sample organism Oryza sativa
Experiment type Non-coding RNA profiling by array
Summary Micro-RNA associated with low-N tolerance have been identified in rice (Oryza sativa) genotypes on the basis of differential expression of miRNA in low-N tolerant and low-N sensitive genotypes of rice. Since, miRNAs emerged as key players in plant responses and adaptation to stress, identification of low-N responsive miRNAs and their targets could help to uncover the molecular mechanisms of N-deficiency tolerance in plants.
We used affymetrix based GeneChip miRNA_2.0 Array to detail the global programme of miRNA expression variation in low-N tolerant (IC-547557) and low -N sensitive (Vivek Dhan).
 
Overall design Low-N tolerant and low-N sensitive rice genotypes were grown using the nutrient medium containing 2 mM K2SO4, 2 mM MgSO4, 1 mM CaCl2, 0.3 mM NaH2PO4, 40 µM Fe-EDTA, 9 µM MnCl2, 25 µM Na2MoO4, 20 µM H3BO3, 1.5 µM ZnSO4, and 1.5 µM CuSO4. The nitrogen in the form of NH4NO3 was maintained as 0.01 mM (low-N condition). The pH of the nutrient solutions was adjusted to 6.0 and the solutions were changed every three days. Twenty-day-old plants were sampled for expression studies of miRNA.
 
Contributor(s) Ahmad A, Nischal L
Citation(s) 23227161
Submission date May 24, 2012
Last update date May 02, 2017
Contact name ALTAF AHMAD
E-mail(s) aahmed@jamiahamdard.ac.in
Phone 911126059688
Fax 91112609663
Organization name Jamia Hamdard (Hamdard University)
Department Department of Botany
Lab Molecular Ecology Laboratory
Street address Faculty of Science
City New Delhi
State/province Delhi
ZIP/Postal code 110062
Country India
 
Platforms (1)
GPL14613 [miRNA-2] Affymetrix Multispecies miRNA-2 Array
Samples (4)
GSM936813 Vivek Dhan Biological replicate 1
GSM936814 Vivek Dhan Biological replicate 2
GSM936815 IC-547557 Biological replicate 1
Relations
BioProject PRJNA167508

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38213_RAW.tar 2.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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