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Series GSE38334 Query DataSets for GSE38334
Status Public on Jul 30, 2012
Title The Translin-TRAX complex/C3PO is a ribonuclease in tRNA processing
Organism Neurospora crassa
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary The endogenous RNA substrates of Translin-TRAX complexes (also known as C3POs) and how they regulate diverse biological processes remain unknown. Here we show that Translin and TRAX do not play a significant role in RNAi in the filamentous fungus Neurospora crassa. Instead, the Neurospora C3PO complex functions as a ribonuclease that removes the 5’ pre-tRNA fragments after the processing of pre-tRNAs by RNase P. In translin and trax mutants, 5’ pre-tRNA fragments accumulate to very high levels that can be degraded specifically by both recombinant and endogenous Neurospora C3PO and recombinant Drosophila C3PO. In addition, the mutants have elevated tRNA levels and increased levels of protein translation and are more resistant to a programmed cell-death inducing agent. Together, this study identified the endogenous RNA substrates of C3PO and provides a potential explanation for its roles in seemingly diverse biological processes.
 
Overall design Examine small RNA population changes in two different strain background
 
Contributor(s) Li L, Gu W
Citation(s) 22773104
Submission date May 30, 2012
Last update date May 15, 2019
Contact name Craig Mello
Organization name University of Massachusetts Medical School
Department Program in Molecular Medicine
Lab Craig Mello
Street address 368 Plantatoin Street, Suite AS5-2047
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platforms (1)
GPL15629 Illumina Genome Analyzer II (Neurospora crassa)
Samples (2)
GSM940260 wt
GSM940261 tsn
Relations
BioProject PRJNA167682
SRA SRP013445

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38334_RAW.tar 2.2 Mb (http)(custom) TAR (of FA)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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