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Series GSE38444 Query DataSets for GSE38444
Status Public on Jun 03, 2015
Title Expression data from murine liver tissue
Organism Mus musculus
Experiment type Expression profiling by array
Summary Our previous studies have linked Trans-fat and monosodium glutamate (MSG) to the development of Nonalcoholic Fatty Liver Disease (NAFLD). We have also shown that MSG can interact with another commonly consumed food additive, aspartame, to impair glucose homeostasis, which is also disrupted in NAFLD. This most recent nutrigenomic study shows how aspartame, MSG and Trans-fat can unfavorably alter hepatic and adipose tissue gene expression.
We used microarray analysis to examine changes in liver gene transcription in response to TFA MSG and/or ASP feeding in male C57Bl/6J mice.
 
Overall design Our study animals were bred from female C57Bl/6J mice fed a standard chow diet until 6 weeks of age whereupon they were placed on one of 4 different dietary regimens for a period of 3 weeks prior to mating. The four dietary regimens used in this study were: [1] TFA diet: consisting of 20% (w/w) Partially Hydrogenated Vegetable Shortening (Test Diet #5C4M containing 8.68% w/w Trans fatty acids; Test Diet Purina, USA), with ad lib drinking water. [2] MSG+TFA diet: TFA diet with ad lib drinking water containing 0.75 g/L of L -Glutamic acid monosodium salt hydrate (MSG catalog G1626 Sigma Aldrich). [3] ASP+TFA diet: (TFA diet with ad lib drinking water containing 0.25 g/L Asp-Phe methyl ester (aspartame, ASP, catalog A5139 Sigma Aldrich). [4] ASP+MSG+TFA diet: TFA diet with ad lib drinking water containing 0.25 g/L aspartame and 0.75 g/L monosodium glutamate. After the 3-week period of adjustment to the respective diets, 15 male offspring were bred, weaned and maintained on these diets for the duration of the study. Offspring were weaned at 4 weeks of age . Liver tissue (15 per diet group) were used at 20 weeks for RNA extraction and hybridization on Affymetrix microarrays.
 
Contributor(s) Collison KS, Makhoul NJ, Zaidi MZ
Citation(s) 23783067
Submission date Jun 04, 2012
Last update date Aug 23, 2019
Contact name Kate S Collison
Organization name King Faisal Specialist Hospital & Research Centre
Street address Department Biological & Medical Research
City Riyadh
ZIP/Postal code POB3354
Country Saudi Arabia
 
Platforms (1)
GPL10740 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [probe set (exon) version]
Samples (12)
GSM942185 male TFA diet liver, biological replicate 1
GSM942186 male TFA diet liver, biological replicate 2
GSM942187 male TFA diet liver, biological replicate 3
This SubSeries is part of SuperSeries:
GSE38446 Expression data from murine liver and adipose tissue
Relations
BioProject PRJNA167971

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE38444_RAW.tar 51.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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