|
| Status |
Public on Feb 07, 2013 |
| Title |
Genome-wide binding of Yki and GAF in embryos and wing imaginal discs [ChIP-Seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The Hippo pathway regulates metazoan growth, acting through the transcriptional co-activators Yorkie (in Drosophila) and Yap and Taz (in vertebrates). Much attention has been focused on upstream regulators of Yorkie and its homologues. In contrast, the mechanisms by which they actually promote transcription have remained poorly understood. Genome-wide chromatin binding experiments support extensive functional overlap between Yorkie and GAF. Chromatin binding identifies thousands of Yorkie sites, the majority of which are associated with elevated transcription, based on genome-wide analysis of mRNA and histone H3K4Me3 modification. Our studies establish a molecular basis for transcriptional activation by Yorkie and implicate it as a global regulator of transcriptional activity in Drosophila.
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| Overall design |
This is a dataset generated by the Drosophila Regulatory Elements modENCODE Project led by Kevin P. White at the University of Chicago. This dataset was generated in collaboration with Ken Irvine at HHMI/Rutgers University and Richard S. Mann at Columbia University. It contains ChIP-seq data (Illumina) for multiple transcription factor antibodies in Drosophila embryos and larval wing imaginal discs.
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| |
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| Contributor(s) |
Slattery M, Oh H, Ma L, Crofts A, Grundstad J, White K, Mann R, Irvine K |
| Citation(s) |
23395637, 25017066 |
| |
| Submission date |
Jun 08, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Kevin P. White |
| E-mail(s) |
kpwhite@uchicago.edu
|
| Organization name |
University of Chicago
|
| Department |
Institute for Genomics and Systems Biology
|
| Street address |
900 E. 57th STR. 10th FL.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60615 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (14)
|
| GSM945885 |
Yki embryo ChIP, replicate1 |
| GSM945886 |
Yki embryo ChIP, replicate2 |
| GSM945887 |
Yki embryo ChIP, replicate3 |
| GSM945888 |
H3K4me3 embryo ChIP, replicate1 |
| GSM945889 |
H3K4me3 embryo ChIP, replicate2 |
| GSM945890 |
Embryo input |
| GSM945891 |
GAF wing disc ChIP, replicate1 |
| GSM945892 |
GAF wing disc ChIP, replicate2 |
| GSM945893 |
Yki wing disc ChIP, replicate1 |
| GSM945894 |
Wing disc input, replicate1 |
| GSM945895 |
Wing disc input, replicate2 |
|
| This SubSeries is part of SuperSeries: |
| GSE43130 |
Genome-wide binding of Yki and GAF in embryos and wing imaginal discs |
|
| Relations |
| BioProject |
PRJNA168202 |
| SRA |
SRP013620 |
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