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Series GSE39078 Query DataSets for GSE39078
Status Public on Oct 01, 2012
Title Cohesin association to replication sites depends on Rad50 and promotes fork restart
Platform organism Saccharomyces cerevisiae
Sample organism Saccharomyces cerevisiae W303
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary The cohesin complex holds together newly-replicated chromatids and is involved in diverse pathways that preserve genome integrity. We show that in budding yeast, cohesin is transiently recruited to active replication origins and it spreads along DNA as forks progress. When DNA synthesis is impeded, cohesin accumulates at replication sites and is critical for the recovery of stalled forks. Cohesin enrichment at replication forks does not depend on H2A(X) formation, which differs from its loading requirements at DNA double-strand breaks (DSBs). However, cohesin localization is largely reduced in rad50delta mutants and cells lacking both Mec1 and Tel1 checkpoint kinases. Interestingly, cohesin loading at replication sites depends on the structural features of Rad50 that are important for bridging sister chromatids, including the CXXC hook domain and the length of the coiled-coil extensions. Together, these data reveal a novel function for cohesin in the maintenance of genome integrity during S phase.
Overall design Scc1 ChIP, Rad50 ChIP and BrdU IP in wild type and mutants at different stages of the cell cycle.
Contributor(s) Tittel-Elmer M, Lengronne A, Davidson MB, Bacal J, François P, Hohl M, Petrini JH, Pasero P, Cobb JA
Citation(s) 22885006
Submission date Jul 03, 2012
Last update date Jan 09, 2016
Contact name Philippe Pasero
Organization name Institute of Human Genetics - CNRS
Lab Pasero
Street address 141 rue de la Cardonille
City Montpellier
ZIP/Postal code 34396
Country France
Platforms (1)
GPL7250 [Sc03b_MR] Affymetrix GeneChip S. cerevisiae Tiling 1.0R Array
Samples (19)
GSM968814 Scc1_WT_HU_60_min, rep1
GSM968815 Scc1_WT_G2_nocodazole
GSM968816 Scc1_WT_Sphase_16°C
BioProject PRJNA170884

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39078_RAW.tar 1.4 Gb (http)(custom) TAR (of BAR, CEL)
Processed data provided as supplementary file

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