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| Status |
Public on Jul 06, 2012 |
| Title |
Expression analysis of Plasmodium falciparum 3D7 delta-PfPuf2 mutant |
| Organism |
Plasmodium falciparum 3D7 |
| Experiment type |
Expression profiling by array
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| Summary |
Investigation of whole genome gene expression level changes in Plasmodium falciparum 3D7 delta-PfPuf2 mutant, compared to the wild-type strain 3D7. The mutation engineered into this strain render tanslational control.
The mutants analyzed in this study are further described in Miao J, Li J, Fan Q, Li X, Li X, Cui L.2010. The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum. J Cell Sci. 123(7):1039-49 (PMID 20197405).
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| Overall design |
A 12 chip study using total RNA recovered from six separate wild-type cultures of Plasmodium falciparum 3D7 at gametocyte stage III (three cultures) and stage V (three cultures) and six separate cultures of dalta PfPuf2 mutant at gametocyte stage III (three cultures) and stage V (three cultures). Each chip measures the expression level of 5,367 genes from Plasmodium falciparum 3D7 with 45-60 mer probes with two replicates on final array of 71618 probes.
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| Contributor(s) |
Jun M, Liwang C |
| Citation(s) |
23637595 |
| |
| Submission date |
Jul 05, 2012 |
| Last update date |
May 16, 2014 |
| Contact name |
JUN MIAO |
| E-mail(s) |
jmiao1@usf.edu
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| Phone |
8139747374
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| Organization name |
University of South Florida
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| Department |
Internal Medicine
|
| Lab |
Jun Miao and Liwang Cui
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| Street address |
3720 Spectrum Boulevard, MDC84
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| City |
Tampa |
| State/province |
Florida |
| ZIP/Postal code |
33612 |
| Country |
USA |
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| Platforms (1) |
| GPL15768 |
NimbleGen P. falciparum 72K array [100712_Pfal_3D7_JM_exp_72K] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA170058 |
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