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| Status |
Public on Jul 04, 2013 |
| Title |
Induced Cdx2 binding in progenitor motor neurons and its effect on H3K27me3 chromatin domains [Affymetrix] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We aim to understand the role that Cdx2 plays in specifying the rostro-caudal identity of differentiating motor neurons. We find that expressing Cdx2 in combination with FGF signaling is sufficient to produce motor neurons with a more caudal identity. ChIP-seq analysis of Cdx2 finds that it binds extensively throughout the Hox regions in progenitor motor neurons. Analysis of polycomb-associated chromatin over Hox regions in the subsequently generated motor neurons finds that Cdx2 binding corresponds to chromatin domains encompassing de-repressed caudal Hox genes. These results suggest a direct role for Cdx2 in specifying caudal motor neuron identity.
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| Overall design |
Expression studies: Affymetrix arrays are used to profile gene expression in ES cells, RA/Hh-derived Day 5 motor neurons, and RA/Hh-derived motor neurons that have also been exposed to Dox (to activate iCdx2) and FGF.
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| Contributor(s) |
Mahony S, Mazzoni EO, Morrison CA, Gifford DK, Wichterle H |
| Citation(s) |
23955559 |
| |
| Submission date |
Jul 17, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Shaun Mahony |
| E-mail(s) |
mahony@psu.edu
|
| Phone |
814-865-3008
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| Organization name |
Penn State University
|
| Department |
Biochemistry & Molecular Biology
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| Lab |
Shaun Mahony
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| Street address |
404 South Frear Bldg
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| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE39453 |
Induced Cdx2 binding in progenitor motor neurons and its effect on H3K27me3 chromatin domains |
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| Relations |
| BioProject |
PRJNA170848 |
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