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Series GSE39422 Query DataSets for GSE39422
Status Public on Jul 04, 2013
Title Induced Cdx2 binding in progenitor motor neurons and its effect on H3K27me3 chromatin domains [Affymetrix]
Organism Mus musculus
Experiment type Expression profiling by array
Summary We aim to understand the role that Cdx2 plays in specifying the rostro-caudal identity of differentiating motor neurons. We find that expressing Cdx2 in combination with FGF signaling is sufficient to produce motor neurons with a more caudal identity. ChIP-seq analysis of Cdx2 finds that it binds extensively throughout the Hox regions in progenitor motor neurons. Analysis of polycomb-associated chromatin over Hox regions in the subsequently generated motor neurons finds that Cdx2 binding corresponds to chromatin domains encompassing de-repressed caudal Hox genes. These results suggest a direct role for Cdx2 in specifying caudal motor neuron identity.
Overall design Expression studies: Affymetrix arrays are used to profile gene expression in ES cells, RA/Hh-derived Day 5 motor neurons, and RA/Hh-derived motor neurons that have also been exposed to Dox (to activate iCdx2) and FGF.
Contributor(s) Mahony S, Mazzoni EO, Morrison CA, Gifford DK, Wichterle H
Citation(s) 23955559
Submission date Jul 17, 2012
Last update date Feb 11, 2019
Contact name Shaun Mahony
Phone 814-865-3008
Organization name Penn State University
Department Biochemistry & Molecular Biology
Lab Shaun Mahony
Street address 404 South Frear Bldg
City University Park
State/province PA
ZIP/Postal code 16802
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM967943 Day0_rep1
GSM967944 Day0_rep2
GSM967945 Day0_rep3
This SubSeries is part of SuperSeries:
GSE39453 Induced Cdx2 binding in progenitor motor neurons and its effect on H3K27me3 chromatin domains
BioProject PRJNA170848

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Supplementary file Size Download File type/resource
GSE39422_RAW.tar 33.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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