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| Status |
Public on Jul 23, 2015 |
| Title |
Mouse bone marrow cells transfected with mmu-miR-142-3p mimic oligo. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Tumor progression is accompanied by an altered myelopoiesis that causes the accumulation of cells inhibiting anti-tumor T lymphocytes. We previously reported that immunosuppressive cells can be generated in vitro from bone marrow cells (BM) after four days GM-CSF and IL-6 treatment. Here, we describe that miR-142-3p down-regulation directs macrophage differentiation and determines the acquisition of their immunosuppressive function in cancer. Enforced miR over-expression impaired monocyte to macrophage transition both in vitro and in vivo. Conversely, forced miR down-regulation promoted the generation of immunosuppressive macrophages even during G-CSF-induced granulocytic differentiation. To identify how miR-142-3p regulates MDSC generation and activity, we analyze the gene expression of BM cultures transfected with either CTRL- or miR 142-3p mimic oligo -transfected before four days GM-CSF and IL-6 treatment.
Keywords: Expression profiling by array
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| Overall design |
BM cells were transfected either CTRL- or miR 142-3p mimic oligo before GM-CSF and IL-6 treatment to generate in vitro MDSCs during enforced miR over-expression. A triplicate of each sample was considered.Total RNA from obtained in vitro BM-differentiated MDSCs was isolated by Trizol reagent, and cRNA samples were hybridized to the Affymetrix GeneChip MOE430 2.0.
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| Contributor(s) |
Bronte V, Sonda N, Simonato F, Peranzoni E, Calì B, Wang E, Naldini L, Gentner B, Trautwein C, Molon B, Bortoluzzi S, Bisognin A |
| Citation(s) |
23809164 |
| |
| Submission date |
Aug 01, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Stefania Bortoluzzi |
| E-mail(s) |
stefania.bortoluzzi@unipd.it
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| Phone |
++30 049 827 6502
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| Organization name |
University of Padova
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| Department |
Department of Molecular Medicine
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| Lab |
Computational Genomics
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| Street address |
via G. Colombo 3
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA171744 |
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