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| Status |
Public on Jan 10, 2006 |
| Title |
Pac1+/+ versus Pac1-/- BMMCs_IgE-DNP 2 h |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Bone marrow-derived mast cells were differentiated over 4-6 weeks using bone marrow from Pac-1+/+ and Pac1-/- littermate mice. Cell purity was 99% c-kit and Fc epsilon receptor positive as assessed by flow cytometry. Cells were stimulated by Fc epsilon receptor crosslinking using IgE-DNP/HSA for sensitization for 18 hours and DNP-HSA antigen for crosslinking for 2 hours. Gene transcript abundance was determined and scaled to 150 using alogorithms in MicroArray Analysis Suite Software 5.0 (Affymetrix).
Keywords: Cpmparison of Pac-1+/+ and Pac-1-/- activated BMMCs
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| Overall design |
Two genechips on independent mBMMC cultures was performed. The signal data of the .CEL files for the four Affymetrix 430A chips (2 Pac1+/+ and 2 Pac1–/–) were normalised using the RMA method 49. The ebayes function implemented in the version 1.3.12 of the Limma package (2003) of Bioconductor was used to analyse the data and P-values were adjusted for multiple testing. After conversion from logged values to the original intensities, the data was filtered to remove probe sets with mean, non-logged, intensities below 100. Transcripts with p-values less than 0.05 were considered different from Pac1+/+.
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| Contributor(s) |
Jeffrey KL, Mackay CR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 06, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Charles Reay Mackay |
| E-mail(s) |
c.mackay@garvan.org.au
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| Phone |
+61-2-92958405
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| Fax |
+61-2-92958404
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| Organization name |
Garvan Institute for Medical Research
|
| Street address |
384 Victoria St
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| City |
Darlinghurst |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (4)
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| GSM91238 |
Wild-type BMMCs_IgE-DNP 2h_rep1 |
| GSM91239 |
Pac1-/- BMMCs_IgE-DNP 2h_rep1 |
| GSM91240 |
Wild-type BMMCs_IgE-DNP 2h_rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE4014 |
Pac1+/+ versus Pac1-/- in macrophages and mast cells |
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| Relations |
| BioProject |
PRJNA104887 |
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