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Series GSE40272 Query DataSets for GSE40272
Status Public on Aug 23, 2012
Title Gene-expression profiling of prostate tumors
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Increasing evidence suggests that prostate cancer is overdiagnosed and overtreated, and prognostic biomarkers would aid in treatment selection. To define prognostic biomarkers for aggressive prostate cancer, we carried out gene-expression profiling of 98 prostate tumors and 52 benign adjacent prostate tissue samples with detailed clinical annotation. We identified 28 transcripts significantly associated with recurrence after radical prostatectomy including NuSAP, a protein that binds DNA to the mitotic spindle. Elevated NuSAP transcript levels were associated with poor outcome in two independent prostate cancer gene-expression datasets. To characterize the role and regulation of NuSAP in prostate cancer, we studied the expression of NuSAP in the LNCaP and PC3 human prostate cancer cell lines. Posttranscriptional silencing of the NuSAP gene severely hampered the ability of PC3 to invade and proliferate in vitro. The promoter region of the NuSAP gene contains two CCAAT boxes and binding sites for E2F. Transient transfection of an E2F1 cDNA and 431bp of the NuSAP promoter demonstrated E2F1 as an important regulator of expression. Deletion of the E2F-binding site at nucleotide 246 negated the effects of E2F1 on NuSAP expression. Electrophoretic mobility shift assays demonstrated that nuclear extracts of cells overexpressing E2F1 bound directly to the E2F-binding site in the NuSAP promoter region. Finally, immunohistochemistry showed a strong correlation between E2F1 and NuSAP expression in human prostate cancer samples. NuSAP is a novel biomarker for prostate cancer recurrence after surgery and its overexpression appears to be driven in part by E2F1 activation.
Overall design disease_state_design
Contributor(s) Gulzar Z
Citation(s) 22349817
Submission date Aug 21, 2012
Last update date Dec 24, 2012
Organization Stanford Microarray Database (SMD)
Phone 650-498-6012
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (4)
GPL9497 SMD Print_1529 Homo sapiens
GPL15971 SMD Print_1392 hos Homo sapiens
GPL15972 SMD Print_1540 hoaf Homo sapiens
Samples (153)
GSM989674 PC 430T
GSM989675 PC 97T
GSM989676 PC 501T
BioProject PRJNA173433

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Supplementary file Size Download File type/resource
GSE40272_RAW.tar 13.7 Mb (http)(custom) TAR
Raw data included within Sample table
Processed data included within Sample table

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