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Series GSE40367 Query DataSets for GSE40367
Status Public on Jan 22, 2015
Title Gene expression analysis of liver and colon cancer primary tumors and metastasis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Enriched tumor epithelium from 61 primary and metastasis tumor specimens was obtained by laser capture microdissection (LCM) as previously described (Boersma et al., 2007). In brief, frozen 8-μm serial sections from OCT-preserved frozen tissues were prepared and mounted on plain, uncharged microscope slides. One Hematoxylin/eosin-stained section of each specimen was reviewed by a pathologist to confirm diagnosis and presence of tumor. The pathologist indicated which representative sections of the tumors should be microdissected. LCM was performed with the Pixcell II LCM system (Arcturus, Mountain View, CA). Total RNA was isolated using the PicoPure protocol (Arcturus, Mountain View, CA). The mRNA was amplified with two linear amplification steps by in vitro transcription using the MEGAscript T7 kit (Ambion, Austin, TX) followed by the labeling step using the BioArray HighYield RNA Transcript Labeling Kit T3 from Enzo Life Sciences (Farmingdale, NY). Labeled cRNA was hybridized onto Affymetix GeneChip HG-U133 Plus 2.0 Arrays.
Overall design 61 primary and metastasis tumor specimens
Contributor(s) Roessler S, Wang XW
Citation(s) 25552933, 27569582
Submission date Aug 24, 2012
Last update date Jul 16, 2019
Contact name Xin Wei Wang
Phone 240-760-6858
Organization name National Cancer Institute
Department Laboratory of Human Carcinogenesis
Lab Liver Carcinogenesis Unit
Street address 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892-4255
Country USA
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (61)
GSM992272 Colon adenocarcinoma with liver metastasis, metastasis tumor GIM-01M
GSM992273 Colon adenocarcinoma with liver metastasis, primary tumor GIM-01P
GSM992274 Colon adenocarcinoma with liver metastasis, metastasis tumor GIM-02M
BioProject PRJNA173769

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40367_RAW.tar 310.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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