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Series GSE40519 Query DataSets for GSE40519
Status Public on Aug 01, 2013
Title Genome-wide GTL1 binding sites in Arabidopsis thaliana
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary To understand how GTL1 regulates cell growth, we first identified its potential direct targets by the chromatin immunoprecipitation followed by the hybridization on an Affymetrix Arabidopsis Tiling 1.0R array (ChIP-chip). To enrich the genomic region bound by GTL1 in vivo, we harvested whole aerial parts of 12-day-old gtl1-1 plants complemented with the pGTL:GTL1:GFP constructs and immunoprecipitated the chromatin fragments associated with GTL1-GFP proteins using antibodies against GFP. After applying a cut-off P-values of 0.001of MAT (Model-based analysis of tiling array), we identified a total number of 3,900 putative immediate target genes that showed consistent binding by GTL1.
Overall design Two IP chips compared to two Input chips.
Contributor(s) Breuer C, Morohashi K, Grotewold E, Sugimoto K
Citation(s) 23143274
Submission date Aug 31, 2012
Last update date Sep 04, 2019
Contact name Kengo Morohashi
Phone 614-688-4954
Organization name The Ohio State University
Department Molecular Genetics
Lab Erich Grotewold
Street address 1060 Carmack Rd
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
Platforms (1)
GPL10977 [At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array
Samples (1)
GSM995520 GTL1 ChIP-chip
This SubSeries is part of SuperSeries:
GSE40520 GTL1 regulates ploidy-dependent cell growth through direct transcriptional repression of APC/CCS52A1 in Arabidopsis
BioProject PRJNA174210

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40519_RAW.tar 156.3 Mb (http)(custom) TAR (of BAR, BED, CEL, TXT)
Processed data provided as supplementary file

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