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Series GSE40563 Query DataSets for GSE40563
Status Public on Dec 18, 2013
Title The conserved SUMO ligase Zip3 protein associates differentially with meiotic double-strand break sites and reveals regional variations in the outcome of meiotic recombination
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by array
Summary In meiosis, an excess number of DNA double-strand breaks (DSBs), the initiating DNA lesion, is formed compared to the number of crossovers, one of their repair products that creates the physical links between homologs and allows their correct segregation. It is not known if all DSB hotspots are also crossover hotspots, or if the ratio between DSB and crossovers varies with the chromosomal location. Here, to systematically investigate variation in the DSB/crossover ratio, we have established the genome-wide map of the Zip3 protein binding sites in budding yeast meiosis. We show that Zip3 associates with DSB sites when these are engaged into repair by crossing over, and that Zip3 binding frequency at DSB reflects its tendency to be repaired as a crossover. We further show that the relative amount of Zip3 per DSB varies with the chromosomal location and identify chromosomal features associated with high or low Zip3 per DSB ratio. Among these is the negative regulation by proximity to a centromere and positive by the proximity to axis-associated sequences. This work opens interesting perspectives to understand the role of these extra DSB that are not frequently used for crossover and our findings may extend to mammals that have a large excess of DSB compared to crossovers.
Overall design ChIP-chip experiment in meiotic diploid SK1 yeast cells - two biological replicates
Contributor(s) BORDE V
Citation(s) 23593021
Submission date Sep 04, 2012
Last update date Feb 15, 2018
Contact name Valerie Borde
Phone 33156246700
Organization name Institut Curie
Department CNRSUMR3244
Street address 26 rue d'Ulm
ZIP/Postal code 75248
Country France
Platforms (1)
GPL4131 Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A)
Samples (7)
GSM996636 ChIP-chip of Zip3-Flag from cells at 3 hr in meiosis
GSM996637 ChIP-chip of Zip3-Flag from cells at 4 hr in meiosis
GSM996638 ChIP-chip of Zip3-Flag from cells at 5 hr in meiosis
BioProject PRJNA174310

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40563_RAW.tar 71.4 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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