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| Status |
Public on Apr 15, 2003 |
| Title |
Heat shock - kin82 mutant and wildtype |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
Wild type and kin82 mutant under heat shock.
Cells grown at 25°C were collected by centrifugation, resuspended in an equal volume of 37°C medium, and returned to 37°C for growth. Samples were collected at 0, 5, 15, 30 and 60 minutes after transfer to 37°C.
Experimental samples were used to generate Cy5-labeled cDNA probes, whereas mRNA reference pools extracted from cultures of the respective strains grown to early log phase under normal conditions, were used to generate Cy3-labeled cDNA probes. Cy5- and Cy3-labeled probes were hybridized together to microarrays printed with PCR-amplified fragments, representing 6280 of the Saccharomyces cerevisiae ORFs.
Keywords: time-course
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| Contributor(s) |
Segal E, Shapira M, Regev A, Pe'er D, Botstein D, Koller D, Friedman N |
| Citation(s) |
12740579 |
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| Submission date |
Apr 15, 2003 |
| Last update date |
Feb 23, 2012 |
| Contact name |
Eran Segal |
| E-mail(s) |
eran@cs.stanford.edu
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| Phone |
650-725-8784
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| URL |
http://cs.stanford.edu/~eran
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| Organization name |
Stanford University
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| Department |
Computer Science
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| Street address |
Gates Building 1A
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
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| Samples (10)
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| GSM6004 |
DBY8778_heat_shock2_0min |
| GSM6005 |
delKIN82_heat shock_60min_repeat |
| GSM6006 |
DBY8778_heat_shock2_5min |
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| Relations |
| BioProject |
PRJNA84953 |
| Supplementary data files not provided |
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