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| Status |
Public on Aug 28, 2006 |
| Title |
The Genome Wide Distribution of Acetylated Histone H4 Remodelled through Human Primary Myoblast Differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by SNP array
SNP genotyping by SNP array
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| Summary |
The simultaneous genotyping of tens of thousands of SNP using SNP microarrays is a very important tool that is revolutionizing genetics and molecular biology. In this work, we present a new application of this technique by using it to assess chromatin immunoprecipitation (CHIP) as a means to assess the multiple genomic locations bound by a protein complex recognized by an antibody. We illustrate the use of this technique with an analysis of the change in histone H4 acetylation, a marker of open chromatin and transcriptionally active genomic regions, which occur during the differentiation of human myoblasts into myotubes. Our results are validated by the observation of a significant correlation between the histone modifications detected and the expression of the nearby genes, as measured by DNA microarrays.
This SuperSeries is composed of the SubSeries listed below.
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| Overall design |
This super series is composed of the following subset series:
GSE4131: Triplicate Affymetrix HGU133A/B expression chips were hybridized to RNA extracted from myoblasts and myotubes.
GSE4132: Triplicate Affymetrix 10K ax 13339 SNP chips were hybridized to DNA from myoblasts and myotubes.
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| Citation(s) |
17868463 |
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| Submission date |
Jan 31, 2006 |
| Last update date |
Aug 10, 2018 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
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| Department |
Cellular and Molecular Medicine
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| Lab |
Ottawa Bioinformatics Core Facility
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| Street address |
501 Smyth Rd.
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| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
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| Platforms (3) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL97 |
[HG-U133B] Affymetrix Human Genome U133B Array |
| GPL3400 |
Affymetrix GeneChip Mapping Pre-Release 10K Array (ax13339) |
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| Samples (21)
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| GSM93884 |
Myobalsts_rep2, chip HG-U133B |
| GSM93885 |
Myoblasts_rep3, chip HG-U133A |
| GSM93886 |
Myoblasts_rep3, chip HG-U133B |
| GSM93887 |
Myotubes_rep1, chip HG-U133A |
| GSM93888 |
Myotubes_rep1, chip HG-U133B |
| GSM93889 |
Myotubes_rep2, chip HG-U133A |
| GSM93890 |
Myotubes_rep2, chip HG-U133B |
| GSM93891 |
Myotubes_rep3, chip HG-U133A |
| GSM93892 |
Myotubes_rep3, chip HG-U133B |
| GSM94529 |
Myoblasts_rep1, chip 10K |
| GSM94530 |
Myoblasts_rep2, chip 10K |
| GSM94531 |
Myoblasts_rep3, chip 10K |
| GSM94532 |
Myoblasts_rep1, chip 10K, after chipping with H4-acetylated chromatin |
| GSM94533 |
Myoblasts_rep2, chip 10K, after chipping with H4-acetylated chromatin |
| GSM94534 |
Myoblasts_rep3, chip 10K, after chipping with H4-acetylated chromatin |
| GSM94535 |
Myotubes_rep1, chip 10K, after chipping with H4-acetylated chromatin |
| GSM94536 |
Myotubes_rep2, chip 10K, after chipping with H4-acetylated chromatin |
| GSM94537 |
Myotubes_rep3, chip 10K, after chipping with H4-acetylated chromatin |
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| This SuperSeries is composed of the following SubSeries: |
| GSE4131 |
Determination of myotube and myoblast expression levels |
| GSE4132 |
Mapping of H4-acetylated chromatin modifications in differentiating human primary myoblasts |
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| Relations |
| BioProject |
PRJNA95013 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE4133_RAW.tar |
66.5 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
| Raw data provided as supplementary file |
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