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Status |
Public on Dec 10, 2012 |
Title |
Genome-wide mapping of the TET3 CXXC domain binding sites |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The TET3 CXXC domain has unique DNA binding properties. It binds to DNA in a cytosine-dependent manner that prefers binding to CpG dinucleotides but is not restricted by the CpG-content, distinct from other well-characterized CXXC domains. To map the TET3 CXXC domain binding sites across the human genome, we purified the GST-tagged TET3 CXXC domain protein and performed the GST pull-down assay using the genomic DNA purified from HEK293T cells. The enriched DNA fragments were then sequenced and aligned to human genome(hg19).
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Overall design |
We used the GST pull-down assay followed by DNA deep sequencing to map the DNA bound by the TET3 CXXC domain in vitro.
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Contributor(s) |
Xu Y, Shi Y, Wu F |
Citation(s) |
23217707 |
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Submission date |
Oct 12, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Feizhen Wu |
E-mail(s) |
wufz@fudan.edu.cn
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Phone |
86-21-54237821
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Organization name |
Fudan Univ, Shanghai, China
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Department |
Institutes of Biomedical Sciences
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Lab |
Epigenetics lab
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Street address |
Dongan Road 131, Rm 511 Mingdao Building
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City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
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Platforms (1) |
GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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Samples (1) |
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Relations |
BioProject |
PRJNA177404 |
SRA |
SRP016092 |