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Series GSE41551 Query DataSets for GSE41551
Status Public on Dec 10, 2012
Title Genome-wide mapping of the TET3 CXXC domain binding sites
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The TET3 CXXC domain has unique DNA binding properties. It binds to DNA in a cytosine-dependent manner that prefers binding to CpG dinucleotides but is not restricted by the CpG-content, distinct from other well-characterized CXXC domains. To map the TET3 CXXC domain binding sites across the human genome, we purified the GST-tagged TET3 CXXC domain protein and performed the GST pull-down assay using the genomic DNA purified from HEK293T cells. The enriched DNA fragments were then sequenced and aligned to human genome(hg19).
Overall design We used the GST pull-down assay followed by DNA deep sequencing to map the DNA bound by the TET3 CXXC domain in vitro.
Contributor(s) Xu Y, Shi Y, Wu F
Citation(s) 23217707
Submission date Oct 12, 2012
Last update date May 15, 2019
Contact name Feizhen Wu
Phone 86-21-54237821
Organization name Fudan Univ, Shanghai, China
Department Institutes of Biomedical Sciences
Lab Epigenetics lab
Street address Dongan Road 131, Rm 511 Mingdao Building
City Shanghai
State/province Shanghai
ZIP/Postal code 200032
Country China
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (1)
GSM1018960 293T40Tet3
BioProject PRJNA177404
SRA SRP016092

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Supplementary file Size Download File type/resource
GSE41551_RAW.tar 29.7 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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