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Series GSE41551 Query DataSets for GSE41551
Status Public on Dec 10, 2012
Title Genome-wide mapping of the TET3 CXXC domain binding sites
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The TET3 CXXC domain has unique DNA binding properties. It binds to DNA in a cytosine-dependent manner that prefers binding to CpG dinucleotides but is not restricted by the CpG-content, distinct from other well-characterized CXXC domains. To map the TET3 CXXC domain binding sites across the human genome, we purified the GST-tagged TET3 CXXC domain protein and performed the GST pull-down assay using the genomic DNA purified from HEK293T cells. The enriched DNA fragments were then sequenced and aligned to human genome(hg19).
 
Overall design We used the GST pull-down assay followed by DNA deep sequencing to map the DNA bound by the TET3 CXXC domain in vitro.
 
Contributor(s) Xu Y, Shi Y, Wu F
Citation(s) 23217707
Submission date Oct 12, 2012
Last update date May 15, 2019
Contact name Feizhen Wu
E-mail(s) wufz@fudan.edu.cn
Phone 86-21-54237821
Organization name Fudan Univ, Shanghai, China
Department Institutes of Biomedical Sciences
Lab Epigenetics lab
Street address Dongan Road 131, Rm 511 Mingdao Building
City Shanghai
State/province Shanghai
ZIP/Postal code 200032
Country China
 
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (1)
GSM1018960 293T40Tet3
Relations
BioProject PRJNA177404
SRA SRP016092

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41551_RAW.tar 29.7 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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