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Series GSE41945 Query DataSets for GSE41945
Status Public on Feb 15, 2013
Title Expression profiles of wildtype and Shox2 knockout embryonic limbs
Organism Mus musculus
Experiment type Expression profiling by array
Summary The development of vertebrate extremities is a complex process which requires a highly coordinated network of different transcriptional activities. The homeodomain transcription factor Shox2 is a key player in limb formation controlling neural, muscular and skeletal development. Here, we compared gene expression profiles of wildtype and Shox2 knockout limbs using microarray experiments to identify Shox2 target genes.
 
Overall design Limbs of E12.5 mouse embryos were dissected, fore- and hindlimbs were pooled and genotyped for RNA extraction. RNA from 3 embryos of 2 different pregnancies (in total 6 embryos) was pooled per genotype (Wildtype and Shox2 Knockout) and compared.
 
Contributor(s) Glaser A, Gretz N
Citation(s) 24347445
Submission date Oct 31, 2012
Last update date Apr 24, 2014
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platforms (1)
GPL8462 Affymetrix Mouse Genome UG 430 2.0 Array [CDF: Mouse4302_Mm_UG_v11.cdf]
Samples (2)
GSM1027966 WT RNA pooled from embryonic murine limb tissue
GSM1027967 KO RNA pooled from embryonic murine limb tissue
Relations
BioProject PRJNA178555

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Supplementary file Size Download File type/resource
GSE41945_RAW.tar 26.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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