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Series GSE42172 Query DataSets for GSE42172
Status Public on Dec 31, 2014
Title Effect of cigarette smoke extract, cisplatin, nicotine and/or ionizing radiation on gene expression in the NCI-H460 human lung large cell carcinoma cell line
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To understand the effect of nicotine on sensitivity of cancer cells to radiation or anti-cancer drugs, NCI-H460 human lung large cell carcinoma cells were treated with 6 Gy ionizing radiation or 1 uM cisplatin after exposure to 5 uM nicotine or cigarette smoke extract. Whole genome expression in total RNA extracted from the treated cells was quantified using the GeneChip™ Human Gene 1.0 ST microarray platform from Affymetrix® (Santa Clara, CA).
 
Overall design Cells were subjected to one of nine types of treatment in triplicate and harvested for RNA extraction after 2 or 24 hours. Thus, 54 RNA samples representing biological triplicates for nine types of treatments and two time-points were obtained and analyzed using microarrays (one array per RNA sample, without technical replication).
 
Contributor(s) Patnaik SK, Romano M, Sobus S, Pundt K, Yendamuri S, Warren G
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Submission date Nov 08, 2012
Last update date Jul 26, 2018
Contact name Santosh Kumar Patnaik
Phone 716-8458364
Organization name Roswell Park Comprehensive Cancer Center
Department Thoracic Surgery
Street address Elm and Carlton Streets
City Buffalo
State/province NY
ZIP/Postal code 14263
Country USA
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (54)
GSM1034057 Control_2h_rep1
GSM1034058 Control_2h_rep2
GSM1034059 Control_2h_rep3
This SubSeries is part of SuperSeries:
GSE42334 Effect of cigarette smoke extract, cisplatin, nicotine and/or ionizing radiation on mRNA and microRNA expression in the NCI-H460 human lung large cell carcinoma cell line
Relations
BioProject PRJNA179230

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42172_RAW.tar 219.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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