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| Status |
Public on Jun 20, 2013 |
| Title |
High throughput sequencing enables detailed analysis of S. cerevisiae DNA replication |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
For rapid characterisation of replication dynamics in wild-type cells from an unperturbed cell cycle we have obtained replicating (S phase) and non-replicating (G2 phase) cells by fluorescence activated cell sorting (FACS) and subjected the DNA to copy number analysis.
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| Overall design |
Measurement of genome replication time for various S. cerevisiae strains. For each strain two samples were analysed: a replicating sample (from S phase) and a non-replicating sample (from G2 phase).
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| Contributor(s) |
Nieduszynski CA, Müller CA, Hawkins M, Retkute R, Blythe M, Malla S, Wilson R |
| Citation(s) |
24089142 |
| |
| Submission date |
Nov 13, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Conrad A Nieduszynski |
| Organization name |
Earlham Institute
|
| Lab |
DNA replication & genome stability
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| Street address |
Norwich Research Park
|
| City |
Norwich |
| ZIP/Postal code |
NR4 7UZ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL16234 |
AB 5500 Genetic Analyzer (Saccharomyces cerevisiae) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA179391 |
| SRA |
SRP017163 |
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