NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE42267 Query DataSets for GSE42267
Status Public on Apr 27, 2013
Title Expression profiling of Ciona intestinalis neurula and mid-tailbud stage embryos expressing altered forms of the transcription factor Ci-Tbx2/3 in the notochord
Organism Ciona intestinalis
Experiment type Expression profiling by array
Summary Despite being found in the notochord of several chordates, the roles of the Tbx2 subfamily of T-box transcription factors in the development of this tissue remain largely unknown. We explored the targets of the only Tbx2 subfamily member in Ciona intestinalis, Ci-Tbx2/3, by expressing mutant forms of the transcriptional regulator using the Ci-Bra promoter region.
We produced a dominant interfering version of Ci-Tbx2/3 (Tbx2/3DBD) through expression of a truncated version consisting only of its DNA-binding domain (DBD) and a constitutive activator form by attaching the T-box of Ci-Tbx2/3 to the VP16 transactivation domain (Tbx2/3VP16). These constructs were introduced into 1-cell stage embryos and grown to the neurula (N) or mid-tailbud (mTb) stage to capture targets regulated throughout notochord morphogenesis.
 
Overall design Ci-Tbx2/3 targets were ascertained using whole-genome custom Affymetrix microarrays to compare the transcription levels of Tbx2/3DBD and Tbx2/3VP16 expressing embryos to wild-type controls (Bra>GFP) at the neurula or mTb stages.
 
Contributor(s) Di Gregorio A
Citation(s) 23674602
Submission date Nov 14, 2012
Last update date Jul 03, 2013
Contact name Anna Di Gregorio
E-mail(s) adg13@nyu.edu
Phone 212-998-9564
Organization name NYU College of Dentistry
Department Basic Science and Craniofacial Biology
Lab Di Gregorio
Street address 345 E 24th Street, room 804A
City New York
State/province NY
ZIP/Postal code 10010
Country USA
 
Platforms (1)
GPL15657 [CINT06a520380F] Affymetrix Ciona intestinalis custom-designed array
Samples (17)
GSM1036463 control at N, replicate 1
GSM1036464 control at N, replicate 2
GSM1036465 control at N, replicate 3
Relations
BioProject PRJNA181103

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42267_RAW.tar 66.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap