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Status |
Public on Aug 01, 2016 |
Title |
Expression data of young panicles from microspore mother cell to meiosis of rice |
Platform organism |
Oryza sativa |
Sample organisms |
Oryza sativa Indica Group; Oryza sativa Japonica Group |
Experiment type |
Expression profiling by array
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Summary |
Male sterility is an important trait in hybrid crop breeding. Thermo-sensitive genic male sterility (TGMS) lines, which are male-sterile at restrictive (high) temperatures but convert to male-fertile at permissive (low) temperatures, have been widely utilized in two-line hybrid rice breeding. However, the molecular mechanism underlying TGMS remains unclear. Here we show that the rice (Oryza sativa L.) thermo-sensitive genic male sterile gene 5 (tms5) locus, which in 2010 was present in cultivars occupying more than 71% (2.3 million hectares) of two-line hybrid rice-growing land in China, confers the TGMS trait through a loss-of-function mutation of RNase ZS1, resulting in failure to mediate mRNA decay of three temperature-responsive ubiquitin fusion ribosomal protein L40 genes (UbL40) genes. RNase ZS1, a member of the evolutionarily conserved endonuclease, processed tRNAs in vitro, but does not do so in vivo due to its localization in the cytoplasm. Defective RNase ZS1 in tms5 plants leads to over-accumulation of UbL401, UbL402 and UbL404 mRNAs at restrictive but not permissive temperatures. Over-expression of UbL401 and UbL404 in wild-type plants caused male sterility, whereas knockdown of UbL401 and UbL404 in tms5 plants partially restored the male fertility at restrictive temperatures. Our results uncover a novel mechanism of RNase ZS1-mediated UbL40 mRNA decay which controls TGMS in rice and has potential applications not only of rice but also of other crops.
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Overall design |
To address whether RNase ZS1 involves in mRNA metabolism, whole-genome microarray was performed using RNA from young panicles of wild type (AnN and ZH11) and tms5 (AnS-1 and Os02g12290iL1) plants grown at permissive and restrictive temperatures.
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Contributor(s) |
Zhuang C, Zhou H, Liu Y, Zhu L |
Citation missing |
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Submission date |
Nov 18, 2012 |
Last update date |
Dec 27, 2017 |
Contact name |
Hai Zhou |
E-mail(s) |
hai_zhou127@126.com
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Phone |
86-20-85280299
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Organization name |
South China Agricultural University
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Department |
College of Life Sciences
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Lab |
SKLCUSA
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Street address |
Wushan
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City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
510642 |
Country |
China |
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Platforms (1) |
GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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Samples (8)
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GSM1038367 |
panicles of AnN plants_low temperature |
GSM1038368 |
panicles of AnS-1 plants_low temperature |
GSM1038369 |
panicles of AnN plants_high temperature |
GSM1038370 |
panicles of AnS-1 plants_high temperature |
GSM1038371 |
panicles of ZH11 plants_low temperature |
GSM1038372 |
panicles of Os02g12290iL1 plants_low temperature |
GSM1038373 |
panicles of ZH11 plants_high temperature |
GSM1038374 |
panicles of Os02g12290iL1 plants_high temperature |
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Relations |
BioProject |
PRJNA181190 |