|
| Status |
Public on Dec 20, 2012 |
| Title |
Phf19 knockdown effect on F9 embryonal carcinoma cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
PCL family protein Phf19/Pcl3 is one of the accessory components of the PRC2 core complex, and Phf19 is highly expressed in murine ES cells and an ES cell-like embryonic carcinoma cell line, F9 cells.
Here we performed microarray analysis of embryonal carcinoma cell line F9 following Phf19 knockdown by shRNA. Knocking down Phf19/Pcl3 in F9 embryonic cells led to derepression of numerous PRC2 direct target genes.
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| |
|
| Overall design |
4 sampels including 2 shRNA vector control cell lines and 2 shPhf19 cell lines were used for RNA extraction and Affymetrix mouse 430 2.0 arrays.
|
| |
|
| Contributor(s) |
Lu R, Cai L, Wang GG |
| Citation(s) |
23273982 |
| |
| Submission date |
Nov 21, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Rui Lu |
| E-mail(s) |
ruilu1@uabmc.edu
|
| Organization name |
Univ of Alabama at Birmingham
|
| Street address |
1824 6th Ave S
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA182015 |
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