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Series GSE4272 Query DataSets for GSE4272
Status Public on Feb 28, 2006
Title GLA and profiling of proteasome, Spt23 and Mga2
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by array
Expression profiling by array
Summary The proteasome can regulate transcription through proteolytic processing of transcription factors and via gene locus binding, but few targets of proteasomal regulation have been identified. Using genome-wide location analysis and transcriptional profiling in Saccharomyces cerevisiae, we have established which genes are bound and regulated by the proteasome, and by Spt23 and Mga2, transcription factors activated by the proteasome. We observed proteasome association with gene sets that are highly transcribed, controlled by the mating-type loci, and involved in lipid metabolism. At ribosomal protein genes, proteasome and RNA polymerase II binding was enriched in a proteasome mutant, indicating a role for the proteasome in dissociating elongation complexes. The genomic occupancies of Spt23 and Mga2 overlapped significantly with the genes bound by the proteasome. Finally, the proteasome acts in two distinct ways, one dependent and one independent of Spt23/Mga2 cleavage, providing evidence for cooperative gene regulation by the proteasome and its substrates.
Keywords: ChIP-chip; genetic modification transcriptional profiling
 
Overall design Six genomic localization analysis (GLA, ChIP2) experiments are represented by 18 samples. Six transcriptional profiling experiments (prof) are also represented. Experiments were generally done in triplicate, with fluors swapped on the third replicate.
 
Contributor(s) Auld KL, Brown CR, Casolari JM, Komili S, Silver PA
Citation(s) 16543154
Submission date Feb 18, 2006
Last update date Mar 16, 2012
Contact name Pamela A Silver
E-mail(s) pamela_silver@hms.harvard.edu
Organization name Harvard Medical School
Department Systems Biology
Lab Pamela Silver
Street address 200 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (3)
GPL3464 UofToronto-yeastORF-6.4k4
GPL3469 UofToronto-yeastORF-6.4k6
GPL3470 UofToronto-yeastORF-6.4k3.48
Samples (35)
GSM97331 Pre1_ChIP2_rep3
GSM97334 Pre1_ChIP2_rep1
GSM97335 Pre1_ChIP2_rep2
Relations
BioProject PRJNA94955

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