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Series GSE42722 Query DataSets for GSE42722
Status Public on Dec 05, 2012
Title miRNA expression profiling in tumor-associated BMDCs induced by mouse ovarian and colon tumor cells
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary miRNA expression profiling in tumor-associated BMDCs induced by tumor cells 1D8 or CT-26 in comparison with those cultured in medium.
Overall design To prepare tumor-associated BMDCs(Bone Marrow-derived Dendritic Cells), murine BMDCs were co-cultured with 1D8 or CT-26 cells in a transwell plate in 1640 medium supplemented with 3% FCS and 1% penicillin and streptomycin for 24 hrs. Total RNAs were isolated using the TRIZOL® Reagent(Invitrogen) according to manufacturer’s instructions.The samples were labeled using the miRCURY™ Array Power labeling kit (Exiqon). miRNA array hybridization was performed, and unbound miRNA Labels were washed away. Microarrays were analyzed using GenePix 4000B scanner and GenePix Pro 6 software (Molecular Devices).
Contributor(s) Yang R
Citation(s) 23355742
Submission date Dec 04, 2012
Last update date Apr 05, 2013
Contact name Yang Rongcun
Phone +86-(22)-23509007
Fax +86-(22)-23502554
Organization name Nankai University
Department School of medicine
Street address 94 Weijin Road, Nankai District
City Tianjin, P.R.China
ZIP/Postal code 300071
Country China
Platforms (1)
GPL16352 miRCURY LNA™ microRNA Array V11.0- hsa, mmu & rno
Samples (3)
GSM1048762 BMDC_Control
GSM1048763 BMDC_ID8
GSM1048764 BMDC_CT-26
BioProject PRJNA182941

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42722_raw_and_processed_data.txt.gz 43.3 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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