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| Status |
Public on Aug 14, 2013 |
| Title |
A Unifying Model for Molecular Determinants of the Pre-selection Vβ Repertoire [ChIP-chip] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Chip-chip from pro-T(DN) cells from Rag1KO mice for H3K27ac, P300 and FAIRE
The primary antigen receptor repertoire is sculpted by the process of V(D)J recombination, which must strike a balance between diversification and favoring gene segments with specialized functions. The precise determinants of how often gene segments are chosen to complete variable region coding exons remain elusive. We have quantified Vβ usage in the pre-selection Tcrb repertoire and report relative contributions of 14 distinct features in shaping their recombination efficiencies, including transcription, chromatin environment, spatial proximity to their DβJβ targets, and quality of recombinase recognition elements. Computational analyses provide a unifying model, revealing a minimal set of eight parameters that are predictive of Vβusage, dominated by chromatin modifications associated with transcription, but largely independent of the precise spatial proximity to DβJβclusters.
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| Overall design |
Rag1KO DN epigenetic landscape at Tcrb
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| Contributor(s) |
Gopalakrishnan S, Predeus A, Artyomov M, Oltz EM |
| Citation(s) |
23918392 |
| |
| Submission date |
Dec 11, 2012 |
| Last update date |
Jan 02, 2014 |
| Contact name |
Eugene M Oltz |
| Organization name |
Washington Univeristy in St.Louis
|
| Department |
Pathology and Immunology
|
| Street address |
660 S Euclid Avenue
|
| City |
St.Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE49234 |
A Unifying Model for Molecular Determinants of the Pre-selection Vb Repertoire |
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| Relations |
| BioProject |
PRJNA183642 |
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